molecular identification of commercialized medicinal plants in southern morocco商业化的分子识别药用植物在摩洛哥南部.pdfVIP

Molecular Identification of Commercialized Medicinal Plants in Southern Morocco 1 . 1. ˚ ¨ 2 1 3 ¨ 1 Anneleen Kool * , Hugo J. de Boer , Asa Kruger , Anders Rydberg , Abdelaziz Abbad , Lars Bjork , Gary Martin4 1 Department of Systematic Biology, Evolutionary Biology Centre, Uppsala University, Uppsala, Sweden, 2 Department of Botany, Stockholm University, Stockholm, Sweden, 3 Laboratory of Biotechnology, Protection and Valorisation of Plant Resources, Faculty of Science Semlalia, Cadi Ayyad University, Marrakech, Morocco, 4 Global Diversity Foundation, Dar Ylane, Marrakech, Morocco Abstract Background: Medicinal plant trade is important for local livelihoods. However, many medicinal plants are difficult to identify when they are sold as roots, powders or bark. DNA barcoding involves using a short, agreed-upon region of a genome as a unique identifier for species– ideally, as a global standard. Research Question: What is the functionality, efficacy and accuracy of the use of barcoding for identifying root material, using medicinal plant roots sold by herbalists in Marrakech, Morocco, as a test dataset. Methodology: In total, 111 root samples were sequenced for four proposed barcode regions rpoC1, psbA-trnH, matK and ITS. Sequences were searched against a tailored reference database of Moroccan medicinal plants and their closest relatives using BLAST and Blastclust, and through inference of RAxML phylograms of the aligned market and reference samples. Principal Findings: Sequencing success was high for rpoC1, psbA-trnH, and ITS, but low for matK. Searches using rpoC1 alone resulted in a number of a