molecular identification of a rare subtype of cryptosporidium hominis in infants in china分子识别的一种罕见的亚型隐孢子虫hominis婴儿在中国.pdfVIP

Molecular Identification of a Rare Subtype of Cryptosporidium hominis in Infants in China 1,2 1 1,2 1,2 Huili Zhu , Jinfeng Zhao , Rongjun Wang , Longxian Zhang * 1The College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou, China, 2 International Joint Research Laboratory for Zoonotic Diseases of Henan, Zhengzhou, China Abstract Two Cryptosporidium isolates from separate infants suffering from diarrhea were obtained from a hospital in Zhengzhou, China and were genotyped by PCR amplification and sequence analysis of the small-subunit ribosomal RNA (rRNA) (SSU rRNA), 70-kDa heat shock protein (HSP70), and actin genes. Further subtyping was performed by PCR amplification and sequence analysis of the 60-kDa glycoprotein (gp60) gene. Both the isolates were identified as Cryptosporidium hominis subtype IdA21, a rare subtype previously found only in a human immunodeficiency virus-infected child in South Africa and another child in Jordan. Citation: Zhu H, Zhao J, Wang R, Zhang L (2012) Molecular Identification of a Rare Subtype of Cryptosporidium hominis in Infants in China. PLoS ONE 7(8): e43682. doi:10.1371/journal.pone.0043682 ´ ´ Editor: Christophe Sola, Institut de Genetique et Microbiologie, France Received February 28, 2012; Accepted July 25, 2012; Published August 24, 2012 Copyright: 2012 Zhu et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Funding: This study was supported in part by funds for the Key National Science and Techno