molecular pathway reconstruction and analysis of disturbed gene expression in depressed individuals who died by suicide干扰基因表达的分子途径重建和分析抑郁的人死于自杀.pdfVIP

Molecular Pathway Reconstruction and Analysis of Disturbed Gene Expression in Depressed Individuals Who Died by Suicide 1 6 4,5 2 3 Vladimir Zhurov , John D. H. Stead , Zul Merali , Miklos Palkovits , Gabor Faludi , Caroline Schild- 1 6 1 Poulter , Hymie Anisman , Michael O. Poulter * 1 Molecular Brain Research Group, Robarts Research Institute, University of Western Ontario, London, Ontario, Canada, 2 Laboratory for Neuromorphology, Hungarian Academy of Sciences and Semmelweis University, Budapest, Hungary, 3 Semmelweis University Hospital, Budapest, Hungary, 4 University of Ottawa Institute of Mental Health Research, Ottawa, Ontario, Canada, 5 Departments of Psychology, Psychiatry and Cellular and Molecular Medicine, University of Ottawa, Ottawa, Ontario, Canada, 6 Department of Neuroscience, Carleton University, Ottawa, Ontario, Canada Abstract Molecular mechanisms behind the etiology and pathophysiology of major depressive disorder and suicide remain largely unknown. Recent molecular studies of expression of serotonin, GABA and CRH receptors in various brain regions have demonstrated that molecular factors may contribute to the development of depressive disorder and suicide behaviour. Here, we used microarray analysis to examine the expression of genes in brain tissue (frontopolar cortex) of individuals who had been diagnosed with major depressive disorder and died by suicide, and those who had died suddenly without a history of depression. We analyzed the list of differentially expressed genes using pathway analysis, which is an assumption- free approach to analyze microarray data. Our analysis revealed that the differentially expresse