monocyte chemoattractant protein-1 secreted by decidual stromal cells inhibits nk cells cytotoxicity by up-regulating expression of socs3单核细胞化学引诱物蛋白1由蜕膜基质细胞分泌抑制nk细胞的细胞毒性socs3的调控表达.pdfVIP

Monocyte Chemoattractant Protein-1 Secreted by Decidual Stromal Cells Inhibits NK Cells Cytotoxicity by Up-Regulating Expression of SOCS3 1 2 2 2 3 2 1 Xiaofei Xu , Qingjie Wang , Biping Deng , Huayang Wang , Zhaogang Dong , Xun Qu , Beihua Kong * 1 Department of Obstetrics and Gynecology, Qilu Hospital, Shandong University, Jinan, Shandong, China, 2 Institute of Basic Medical Sciences, Qilu Hospital, Shandong University, Jinan, Shandong, China, 3 Department of Clinical Laboratory, Qilu Hospital, Shandong University, Jinan, Shandong, China Abstract Background: Decidual stromal cells (DSCs) are of particular importance due to their pleiotropic functions during pregnancy. Although previous research has demonstrated that DSCs participated in the regulation of immune cells during pregnancy, the crosstalk between DSCs and NK cells has not been fully elucidated. To address this issue, we investigated the effect of DSCs on perforin expression in CD56+ NK cells and explored the underlying mechanism. Methodology/Principal Findings: Flow cytometry analysis showed perforin production in NK cells was attenuated by DSC media, and it was further suppressed by media from DSCs pretreated with lipopolysaccharide (LPS). However, the expression of granzyme A and apoptosis of NK cells were not influenced by DSC media. ELISA assays to detect cytokine production indicated that monocyte chemoattractant protein-1 (MCP-1) in the supernatant of DSCs conditioned culture significantly increased after LPS stimulation. The inhibitory effect of DSC media on perforin was abolished by the administration of anti-MCP-1 neutralizing antibody. Notably, reduced perforin expression attenua