Definite Focus from Carl Zeiss BioTechniques（明确的重点从卡尔蔡司生物学技术）.pdf

Definite Focus from Carl Zeiss How to Avoid Drifting In this context LSM and TIRF microscopy extend the Particularly, long-term experiments in Live Cell Imaging available options via improved resolution or a better sig- are often impaired or only possible to limited extent as nal-to-noise ratio, respectively. Multichannel time-lapse a result of drifting in z accompanied by the loss of the experiments, in which several different fluorophores in original observation plane. The primary reason for this the cell are observed for a longer period of time, are typi- is the mechanical expansion of the components in the cal. Incubation components for optimal live cell condi- heat-up phase. Definite Focus from Carl Zeiss is able to tions, highly sensitive cameras for the detection of ex- rapidly compensate for this drifting. This is achieved by tremely weak signals, and, in part, very complex software continuous monitoring of the distance between the ob- modules for analysis of the data round out such an imag- jective and the culture vessel by means of infrared light ing station. and a corresponding correction in case of deviations. Definite Focus overcomes the disadvantages of previ- Conventional Methods of Optimisation ous approaches to drift reduction and additionally allows Relief is possible in some cases by means of extensive novel experiments, which e.g., require rapid t