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Compatibility Testing:兼容性测试
Compatibility Testing Ahmad Shihada Silmi Msc, FIBMS IUG What is compatibility testing? Also called pretransfusion testing Purpose: To select blood components that will not cause harm to the recipient and will have acceptable survival when transfused If properly performed, compatibility tests will confirm ABO compatibility between the component and the recipient and will detect the most clinically significant unexpected antibodies Compatibility testing? There are several components of compatibility testing Proper specimen collection Reviewing patient transfusion history ABO, Rh, and antibody testing (screen/ID) Crossmatching Actual transfusion Compatibility testing Can be divided into 3 categories: Preanalytical procedures Serological testing Postanalytical procedures Pre-analytical phases Patient identification Specimen collection Review of patient history Patient Identification Must confirm recipient’s ID from bracelet ON the patient Full patient name and hospital number Name of physician Sample Identification The sample should also have the full patient name, hospital number, and physician Date and time of collection, phlebotomist’s initials All of this should be on the request form and the sample Specimen Tubes Specimen Collection Collected in tube with EDTA or no additives If the venipuncture causes hemolysis, the sample may be rejected True hemolysis in the patient is the result of complement activation Samples are labeled at the bedside (pre-labeling is not recommended) A record of individuals who collect (or test) the specimens should be documented in order to “backtrack” in case of an error Specimen Collection If the sample is drawn from an IV line, the IV infusion should be stopped 5-10 minutes prior to blood drawing and the first 10 mL discarded Testing should be performed on samples less than 72 hours or else complement dependent antibodies may be missed (complement can become unstable) Getting the history Look at recipient’s records for any prior un
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