固相微萃取条件优化和发酵菜粕风味物质分析.doc

固相微萃取条件优化及发酵菜粕风味物质分析 杜静1 钮琰星2* 周琦3 张南南3 李坤鹏3 （怀化学院生物与食品工程学院 民族药用植物资源研究与利用湖南省重点实验室1，怀化 418000） （海南大学 食品学院2，海口 570228） （中国农业科学院油料作物研究所 油料脂质化学与营养湖北省重点实验室，武汉 430062） 摘 要 旨在研究固相微萃取气质联用研究发酵菜粕的风味物质比较不同菜粕发酵前后风味的变化，性DCP) 萃取头，温度70 ℃，吸附30 min，样品量2 g，其中萃取头和吸附温度显著影响萃取效率。发酵前后菜粕的挥发性组分及含量变化都较大，发酵后风味物质的总量都显著增加，硫苷的含量明显降低，表明发酵不但显著增加了风味物质的含量改变了风味，而显著降低了抗营养物质硫苷的含量。 关键词 发酵菜粕 固相微萃取 风味物质 气质联用 中图分类号：TS229 文献标识码：A 文章编号：1003-0174（ ） - - Optimization of solid phase microextraction (SPME) and analysis of volatile compounds in fermented rapeseed meal Du Jing1 Niu Yanxing2* Zhou Qi3 Zhang Nannan3 Li Kunpeng3 (College of Biological and Food Engineering Huaihua University Key Laboratory of Research and Utilization of Ethnomedicinal Plant Resources of Hunan Province1, Huaihua 418000) (College of Food Science Hainan University2, Haikou 570228) (Oil Crops Research Institute of Chinese Academy of Agricultural Sciences Hubei Key Laboratory of Oilcrops Lipid Chemistry and Nutrition3, Wuhan 430062) Abstract The objective of this study was to investigate the optimization of solid phase microextraction (SPME) conditions for fermented rapeseed meal and compare the major volatile compounds and glucosinolate of three cultivars rapeseed meal and fermented rapeseed meal. The effects of type of SPME fiber, adsorption temperature, adsorption time and sample amount were studied to develop SPME conditions for obtaining the highest extraction efficiency. The results showed that optimal conditions were SPME fiber 50/30 μm DVB/CAR/PDMS (DCP), adsorption temperature 70 °C, adsorption time 20 min and sample amount 2 g. The extraction efficiency significantly increased with the increased of adsorption temperature. The species and amount of glucosinolate of fermented rapeseed meal were significantly lower than rapeseed meal, thus microbial fermentation not only improved the favor of rapeseed meal, but also decreased the content of glucosinolate. Key words Fermented rapeseed, solid