艾维茵鸡与固始鸡消化系统组织学的光镜和电镜的观察研究1.PDF

艾维茵鸡与固始鸡消化系统组织学的光镜和电镜的观察研究1 1 1*2 1 1 1 孙桂荣 ，康相涛 ，焦玉萍 ，田亚东 ，李国喜 ， （河南农业大学牧医工程学院，河南，郑州，450002；2、河南省家禽种质资源工程研究中心，河南，郑州，450002） 摘 要 将固始鸡纯系、艾维茵鸡笼养育雏至4 周龄，再笼养或放牧饲养至18 周龄。分别测定4 周龄和18 周龄各 组鸡的胃蛋白酶、小肠中总蛋白水解酶、胰蛋白酶、淀粉酶、脂肪酶和盲肠中纤维素酶活性。结果表明：4 周龄时， 各组鸡的消化酶活性无显著差异（p0.05）。18 周龄时，放养固始鸡纯系纤维素酶显著高于艾维茵纤维素酶的活性， 其他消化酶间差异不显著。光学和电子显微镜的观察结果表明：两种鸡肠段的横截面和纵切面的组织形态结构在光 学显微镜下无太大的差异，腺胃在电子显微镜下无明显差异，而十二指肠、空回肠、盲肠和直肠肠黏膜的表面在电 子显微镜下都有不同程度的差异。 关键词：固始鸡，艾维茵鸡，酶活性，电镜和光镜，消化系统 Comparative Study on digestive system of Gushi chick and Avin broiler chick with light and scanning electron microscopy SUN Gui-rong,KANGXiang-tao,JIAO yu-ping,TIAN Ya-dong,LI Guo-xi (1 College of Animal Husbandry And Veterinary, Henan Agricultural University, Zhengzhou 450002, China;2,Henan Innovative Rearch Center for Poultry Germplasm Resource Zhengzhou 450002,) Abstract: in this experiment,pure lines of Gushi chicks, Avin broiler chicks are raised to 4 weeks old under the same condition, 5 weeks to 18 weeks was free-range reared. slaugher qualities were measured in the end of 18 weeks.The activity of pepsin ,trypsin, total proteinase,a -amylase ,lipase and cellulase in the stomachal , small intestinal and caecum contents ,the length of duodenum ,jejunum-ileum,caeca and rectum were measured respectly on the end of 4 weeks and 18 weeks.furthermore we observe gland paunch tissue morphology structure of small instinal ,caeca and rectum of gushi pure lines and avin broiler chicks utilizing optical and electronic microscope .The results showed that there were no significent difference among each group in the 4 weeks old (p0.05). in the 18 weeks old ,the activity of 3 breeds Gushi chicks in the (FRR) w