紫薯花青素的提取及其在VC含量测定中的应用-食品科学.PDF

134 2016, Vol.37, No.09 食品科学 ※基础研究 紫薯花青素的提取及其在VC含量测定中的应用 郭彩华，卢珍华，伍 菱，陈昭华，陈庆绸，熊何健* （集美大学食品与生物工程学院，福建厦门 361021 ） 摘  要：从紫薯中提取花青素，该花青素在530 nm波长处有特征吸收峰，分子质量范围在850～1 020 u之间。利用 紫薯花青素替代GB/T 6195—1986 《水果、蔬菜维生素C含量测定法（2,6-二氯靛酚测定法）》测定夏橙和西红柿中 VC含量。结果表明：夏橙和西红柿中VC 的含量分别为（25.33 ±0.34 ）、（20.41 ±0.32 ） mg/100 g ；紫薯花青素法 的加标回收率为95.4%～98.6%，相对标准偏差（relative standard deviation ，RSD ）为1.18%～1.22%；2,6-二氯靛酚 法的加标回收率为96.5%～98.7%，RSD为0.86%～0.88% 。建立的利用紫薯花青素替代GB/T 6195—1986中2,6-二氯 靛酚测定水果、蔬菜中VC含量的方法具有安全、廉价的特点，其准确度与2,6-二氯靛酚法滴定法相当。 ： 关键词 紫薯；花青素；GB/T 6195—1986；2,6-二氯靛酚；VC Anthocyanins from Purple Sweet Potato (Ipomoea batatas) and Its Application in VC Quantification * GUO Caihua, LU Zhenhua, WU Ling, CHEN Zhaohua, CHEN Qingchou, XIONG Hejian (College of Food and Biological Engineering, Jimei University, Xiamen 361021, China) Abstract: Anthocyanins were extracted from purple sweet potato (Ipomoea batatas) using an environmentally friendly process. The anthocyanins exhibited characteristic absorption peak at 530 nm, having a molecular weight in the range of 850‒1 020 u. The content of vitamin C in Valencia orange and tomato was determined by a new titration method using the anthocyanins extracted from purple sweet potato in place of 2,6-dichloroindophenol as described in the Chinese national ± ± standard GB/T 6195 －1986. The results showed that vitamin C was (25.33 0.34) mg/100 g in Valencia orange and (20.41 0.32) mg/100 g in tomato. The spiked recoveries of VC with purple sweet potato anthocyanins ranged from 95.4% to 98.6%, wit