天祝白牦牛KAP11基因的克隆及生物信息学分析-广东农业科学.PDF

广东农业科学 2015 年第 2 期 131 天祝白牦牛KAP1.1 基因的克隆 及生物信息学分析 张良斌 潘和平 西北民族大学生命科学与工程学院袁 甘肃兰州 730030冤 要 以牦牛的角蛋白关联蛋白 1.1(KA P1.1)基因为研究对象袁根据 GenBank 收录的黄牛 KA P1.1 基因 的mRNA 序列(登录号院NM _ 001105369.1)设计特异性引物袁通过反转录 PCR 尧PCR 以及克隆方法首次获得牦牛 的KA P1.1 完整 CDS 区袁将所得序列提交到 NCBI 袁登录号为 KJ095199 袁并对其进行生物信息学分析遥 结果表 明袁牦牛 KA P1.1 基因的 CDS 区全长为 501 bp 袁共编码 166 个氨基酸曰编码的蛋白质属于亲水性蛋白质遥 二级 结构含有 琢 螺旋区尧 延伸链尧茁 转角和无规卷曲4 种遥 氨基酸序列与黄牛的同源性最高袁 具有 High sulphur keratin-associated protein 家族蛋白的完整结构域遥 关键词 牦牛曰 KAP1.1 基因曰 绒毛曰 CDS 区 + 中图分类号 S823.8 5;Q785 文献标识码 A 文章编号 1004-874X 2015 冤02-0131-06 Cloning and bioinformatics analysis of KAP1.1 gene in Tianzhu white yak ZHA NG Liang-bin 袁 PAN He-ping ( 730030, ) Life Science and Engineering College , Northwest Unive rs ity for Nationalities , Lanzhou China A bstract: Taking Keratin associated protein 1.1 (KA P1.1) gene of yak as the research obj ect, a specific primer was designed according to the mRNA sequence of cattle KA P1.1 gene (Accession No. NM _ 001105369.1) . Yak 爷s complete CDS sequences of KA P1.1 were obtained by RT -PCR, PCR and cloning, the sequences were submitted to NCBI (Accession No. KJ095199) and analyzed by bioinformatics. The results showed that, the length of CDS sequences in yak KA P1.1 gene was 501 bp encoding 166 amino acids w hic