沙利度胺对双膦酸盐相关颌骨坏死的影响.PDF

·1084 · doi 10.3969/j.issn. 1673-4254.2015.08.02 J South Med Univ, 2015, 35(8): 1084-1089 基础研究 沙利度沙利度胺对双膦酸盐相关颌骨坏死的影响胺对双膦酸盐相关颌骨坏死的影响 1 1 1 1 2 1 宋志强 ，董 伟 ，阴露佳 ，刘娟娟 ，孙 红 ，戚孟春 1 2 华北理工大学 口腔医学院口腔颌面外科，基础医学院病理教研室，河北 唐山 063000 摘要：目的研究沙利度胺对双膦酸盐相关颌骨坏死的影响。方法 36 只大鼠随机分成A、B、C 组，分别接受生理盐水、唑来膦 酸、唑来膦酸+沙利度胺治疗。用药3周后，拔除大鼠左上颌第1磨牙。拔牙后4、8周，收获标本，评价颌骨坏死、微血管生成及细 胞凋亡情况。结果 拔牙后4、8周，大鼠上颌骨拔牙创处均无死骨裸露，但B 组、C 组部分标本可见一些小的瘘管。组织学检查 显示，A 组标本未见死骨，而B 组、C组在拔牙窝周围可见小块死骨。B 组、C组拔牙窝周围区域空骨陷窝百分比和死骨面积显著 多于A 组（P0.01），而骨陷窝密度则显著下降。B 组、C 组微血管密度较A 组也显著降低，在4 周时分别下降了和25.87%和 55.27 %（P0.01），在8 周时分别下降了45.62% 和72.84%（P0.01）；凋亡细胞数则在4 周时分别增长了54.80%和87.89%（P 0.01），在8周分别增长了208.08% 和250.58%（P0.01）。结论 沙利度胺加重了唑来膦酸诱发的早期阶段的颌骨坏死；沙利度 胺和唑来膦酸对颌骨坏死的作用与微血管生成抑制有关。 关键词：唑来膦酸；血管生成；双膦酸盐；颌骨坏死；沙利度胺 Effect of thalidomide on development of bisphosphonate-related osteonecrosis of the jaws in rats 1 1 1 1 2 1 SONG Zhiqiang , DONG Wei , YIN Lujia , LIU Juanjuan , SUN Hong , QI Mengchun 1Department of Oral and Maxillofacial Surgery, College of Stomatology, 2Department of Pathology, College of Basic Medicine, North China University of Science and Technology, Tangshan 063000, China Abstract: Objective To investigate the effect of thalidomide on the development of bisphosphonate-related osteonecrosis of the jaws (BRONJ). Methods Thirty-six rats were randomly divided into groups A, B and C, and treated with saline, zoledronate and zoledronate plus thalidomide, respectively. Three weeks later, the left maxillary first molars of the rats were extracted. Four and eight weeks after tooth extraction, samples were harvested for evaluation of osteonecrosis of the jaws, microvessel density, and cell apoptosis. Results At both of the time points, no expos