1,25-二羟维生素D3抑制大鼠角膜移植术后免疫排斥反应的实验研究.docVIP

1,25-二羟维生素D3抑制大鼠角膜移植术后免疫排斥反应的实验研究 胡雁，周善璧 （重庆医科大学附属第一医院眼科，重庆，400016） 作者简介：胡雁（1981－），女，重庆人，硕士，住院医师，主要从事眼表面疾病研究工作。电话通讯作者：周善璧，zhoushanbi@ 提要：目的 研究1,25-二羟维生素D3对角膜移植术后免疫排斥反应的影响。方法 以SD大鼠为供体，Wistar大鼠为受体建立穿透性角膜移植模型，实验组及实验对照组行同种异体角膜移植，对照组行自体角膜移植；术后0-13d实验组腹腔注射1,25-二羟维生素D3 1.0μg ·kg·d；对照组和实验对照组注射灭菌花生油2ml/d；术后观察植片存活情况，并于术后14d、21d、30d每组随机处死5只大鼠进行植片病理学检查和ELISA检测外周血IL-1β、IL-2、IL-8、IL-10。结果 对照组角膜植片平均存活时间为（21.7±6.8）d，实验对照组为（11.2±2.5）d，实验组为（19.3±5.2）d，三组比较统计学差异非常显著（P0.01）；病理学检查示实验组淋巴细胞浸润和新生血管均较实验对照组少；实验组外周血中IL-1β含量与对照组相比下，含量（P0.0）。1,25-二羟维生素D3能在角膜移植后有效的保护植片免受免疫损伤，抑制排斥反应，延长植片存活时间。 关键词 ：1,25-二羟维生素D3；角膜移植；白介素 中图法分类号：R772.2 Experimental study on the effects of 1, 25-dihydroxyvitamin D3 of rat corneal allograft rejection HU Yan, ZHOU Shan-bi (Department of Ophthalmology, the First Affiliated Hospital, Chongqing Medical University, Chongqing 400016, China) Abstract: Objective To investigate the effects of 1, 25-dihydroxyvitamin D3 on prevention of corneal allograft rejection. Methods SD rats were used as recipients, and Wistar rats were used as donors. SD rats were randomly divided into three groups: Autograft control group; Allograft control group (the control groups were given placebo only); Allograft group were treated with 1, 25-dihydroxyvitamin D3(1.0?g·kg·d).The drugs were delivered for 14 days beginning at the day of transplantation. Each rat was examined by operating microscopy everyday after transplantation. 5 SD rats in each group were killed respectively at the 14days, 21days, and 30days postoperatively. Neovascularization and inflammation were evaluated with HE staining. ELISA assay was used to detect the contents of IL-1β, IL-2, IL-8, and IL-10 in the peripheral blood. Results the mean survival time of the autograft control group was （21.7±6.8）droup was merely（11.2±2.5）d roup led to a statistically significant prolongation of the mean survival time to（19.3±5.2）droup has less neovascularization and inflammation than the allograft control group; the level of express