201308193论着瘦素对糖尿病心肌病大鼠心室重构的影响.docVIP

201308193 论著 余帆，徐彤彤（541001桂林，广西桂林市桂林医学院附属医院特需病区） [摘要]目的观察瘦素对糖尿病性心肌病（diabetic cardiomyopathyDCM）大鼠心室重构的影响。方法10只SPF级健康SD大鼠8周龄170～190g）作为正常对照组（NC组），予以普通饲料喂养。另0 SPF级健康SD大鼠8周龄170～190g）采用高糖高脂喂养后2次腹腔注射链脲佐菌素（STZ）的方法建立DCM大鼠模型，将DCM大鼠随机分为A组、瘦素50 μg/kg组（B组）、瘦素100 μg/kg（C组）。在第11周时，B组、C组分别给予每日腹腔注射瘦素50、100 μg/kg，而NC组与A组均注射等量蒸馏水，持续5第12周末检测组空腹血糖、胰岛素、瘦素及血管紧张素Ⅱ（Angiotensin Ⅱ，AngⅡ）水平；心脏彩超测定大鼠心脏左室舒张末期内径（left ventricular internal diameter at end-diastoleLVIDd）、左室舒张末压（left ventricular end-diastolic pressureLVEDP）、左室射血分数（left ventricular ejection fractionLVEF）。结果与NC组相比，A组出现心功能减退，其瘦素、胰岛素及AngⅡ水平升高；不同剂量瘦素干预后，B组与C组大鼠心功能各项指标变化显著，心功能减退更为严重，瘦素、胰岛素及AngⅡ水平显著升高，且C组变化程度显著高于B组。结论瘦素可能通过神经内分泌系统参与了糖尿病心肌病的发生发展，且与胰岛素抵抗、肾素-血管紧张素系统等密切相关。[关键词] 瘦素；糖尿病心肌病；胰岛素抵抗；血管紧张素Ⅱ Effects of Leptin on Ventricular Remodeling in Diabetic Cardiomyopathy Rats YU Fan， XU Tong-tong（VIP ward， Affiliated Hospital of Guilin Medical College，Guilin 541001，China.） Abstract]Objective To establish the diabetic cardiomyopathy rat model， thenobserve the effects of leptin on ventricular remodeling in diabetic cardiomyopathy rats. Methods Ten healthy male SD rats (SPF， 8 weeks，weighing 170 ~ 190g) wereselected as normal control group (NC)， those who were given normal diet. Then other fifty healthy SD rats (SPF， 8 weeks，weighing 170 ~ 190g) were intraperitoneally injected twicewith streptozocin(STZ) to induce diabetic cardiomyopathy. The diabetic cardiomyopathy rats were randomly divided into three groups：the DCM group（n=10the 50μg/kg leptin group(n=10) and the 100μg/kg leptin group(n=10). The diabetic cardiomyopathy rats were fed on high fat/high sucrose diet. In the eleventh weeks， the latter two groups were respectively given daily intraperioneal injections of leptin on 50μg/kg and 100μg/kg， while the NC and A groups were injected with distilled water for 5 days.Serum IL-6，Ang-Ⅱand LVEDP，LVIDd， LVEF were analysed after 12 weeks later.ResultsThere were abnormal cardiac function in DCM rats. Serum IL-6 and Ang-Ⅱin DCM groups was significant