AMD3100对Lewis肺癌进展及荷瘤鼠脾脏髓源抑制性细胞积聚的影响.docVIP

AMD3100对Lewis肺癌进展及荷瘤鼠脾脏髓源抑制性细胞积聚的影响 董伯升，解瑞玲，庞 慧，卞俊杰，郭伟华，成 媛，李醒亚(450052 郑州，郑州大学第一附属医院肿瘤科) [摘要] 目的 观察趋化因子受体CXCR4拮抗剂AMD3100对Lewis肺癌进展及荷瘤鼠脾脏髓源抑制性细胞（myeloid derived suppressor cells ，MDSCs）积聚的影响。方法 60只荷Lewis肺癌C57BL/6小鼠分为2组：PBS组（腹腔只注射PBS液0.2ml/只）、AMD3100组[腹腔注射溶解有2 mg/（kg·d) AMD3100的PBS液0.2 ml/只，1次/d，共26 d）分组法处死2组荷瘤鼠各15只，剥离瘤体称量，观察肺转移灶数目，用免疫组化法计数瘤组织微血管密度（MVD），流式细胞仪测小鼠脾脏MDSCs比例；统计2组剩余小鼠自然生存天数。结果 PBS组、AMD3100组小鼠Lewis肺癌成瘤时间分别为（8.03±1.47）、（9.97±1.50））小鼠, 近交C57BLffects of AMD3100 on progression of Lewis lung carcinoma and accumulation of myeloid derived suppressor cells in tumor-bearing mice’s spleen Dong Bosheng, Xie Ruiling, Pang Hui, Bian Junjie, Guo Weihua, Cheng Yuan, Li Xingya (Department of Oncology, First Affiliated Hospital, Zhengzhou University, Zhengzhou, Henan Province, 450052, China) [Abstract] Objective To investigate the effects of AMD3100, a specific antagonist of chemokine receptor-4, on the progression of Lewis lung carcinoma and the accumulation of myeloid derived suppressor cells (MDSCs) in tumor-bearing mice’s spleen. Methods Sixty C57BL/6 mice, inoculated subcutaneously Lewis lung carcinoma cell line LLC, were separated into 2 groups (n=30), the PBS and AMD3100 group. The mice in the 2 groups were intraperitoneally injected with 0.2 ml PBS solution or 0.2 ml PBS solution dissolved with AMD3100 [2 mg/(kg·d) ] separately. All the mice were injected once per day, and the injection was lasted for 26 d. The mice’s weight were measured every other day and the time of oncogenesis was recorded. In 26 d later, 15 mice in every group were randomly sacrificed to count the number of pulmonary metastatic nodules and the weight of Lewis lung carcinoma. Flow cytometry and immunohistochemistry were used to detect the percentage of MDSCs in mice’s spleen and the tumor’s microvessel density (MVD) separately. Survival time of the remaining mice in the 2 groups were recorded. Results The time of oncogenesis in PBS and AMD3100 group were（8.03±1.47） and （9.97±1.50） d separate