COX-2抑制剂在大鼠胰十二指肠移植缺血再灌注损伤中对P选择素和细胞间粘附分子-1的影响.docVIP

COX-2抑制剂在大鼠胰十二指肠移植缺血再灌注损伤中对P选择素和细胞间粘附分子-1的影响 赵雪云，张 力，刘胜春 （400016 重庆,重庆医科大学附属第一医院内分泌外科） [摘要] 目的 探讨特异性环氧合酶-2抑制剂（cyclooxygenase-2 inhibitor，COX-2 inhibitor）NS398能否抑制大鼠胰十二指肠移植缺血再灌注损伤中P选择素（P-selectin，Ps）及细胞间粘附分子-1（intercellular adhesion molecule-1，ICAM-1）的表达。方法 采用单纯随机抽样的方法将75只SD大鼠分为3组：假手术组（15只）、移植组（15对）、移植+NS398组（15对）。各组均于术后1、3、6 h取血测定血清淀粉酶含量，光镜下观察胰腺病理改变，免疫组织化学法和RT-PCR法分别检测胰腺组织中ICAM-1及Ps的表达情况和基因水平的变化。结果 假手术组各时间点胰腺组织损伤不明显，血淀粉酶不升高（P＞0.05）；随再灌注时间的延长，移植组胰腺组织损伤加重，血淀粉酶较同时点假手术组明显升高（P＜0.05）；但与移植组比较，移植+NS398组胰腺组织损伤及血淀粉酶升高均较轻（P＜0.05）。假手术组各时点Ps和ICAM-1均不表达；移植组、移植+NS398组各时点Ps和ICAM-1均有表达，但移植+NS398组Ps和ICAM-1的表达均明显低于移植组（P＜0.05）。结论 NS398能抑制胰十二指肠移植缺血再灌注损伤大鼠的Ps及ICAM-1表达，对大鼠胰十二指肠移植缺血再灌注损伤有保护作用。 [关键词] 胰腺移植；缺血再灌注损伤；环氧合酶-2抑制剂；P选择素；细胞间粘附分子-1 [中图法分类号] [文献标志码] A [基金项目] 国家自然科学基金 [通信作者] 刘胜春，E-mail: liushengchun1968@163.com Influence of COX-2 inhibitor on P-selectin and ICAM-1 in rats with ischemia-reperfusion injury following pancreaticoduodenal transplantation Zhao Xueyun, Zhang Li, Liu Shengchun (Department of Endocrine Surgery, First Affiliated Hospital of Chongqing Medical University, Chongqing, 400016, China) [Abstract] Objective To investigate whether NS398 which is the specific cyclooxygenase-2 inhibitor can inhibit P-selectin and ICAM-1 in rats with ischemia-reperfusion injury following pancreaticoduodenal transplantation. Methods Seventy-five Sprague-Dawley rats were divided into three groups with the method of simple random sampling. Group A: sham operation group (fifteen rats), group B: transplanted group (thirty rats), group C: transplanted with NS 398 group (thirty rats). Blood samples in the three groups were harvested at 1, 3 and 6 hours after the operation for serum amylase assay. Pancreas samples were harvested at the same time for morphological study by light microscopy. The expression of Ps and ICAM-1 gene and protein of pancreas were analyzed by RT-PCR and immunohistochemical method. Results The level of serum amylase and the