Cyr61在人肿瘤组织中的表达.docVIP

Cyr61在人肿瘤组织中的表达 李志强，司维柯，丁 伟，李 军，潘 静 （400038重庆，第三军医大学医学检验系临床血液学教研室） 【摘 要】 目的 观察分析Cyr61在人类肿瘤组织中的表达情况，为进一步探讨Cyr61是否可作为新的肿瘤诊断标志物提供实验数据支持。方法 采用HE染色、免疫组化的方法检测多组织肿瘤组织芯片和30例肿瘤组织标本 (包括食道癌、胃癌、直肠癌、肺癌、乳腺癌和肝癌组织标本各5例)中Cyr61的表达。结果 组织芯片中77%（38/48）的肿瘤组织Cyr61表达阳性，93%（28/30）肿瘤组织标本中Cyr61表达阳性。其中神经、食管和甲状腺等肿瘤组织中呈强阳性表达，胃、肝、结肠、直肠、肺、肾、子宫、皮肤、卵巢、乳腺等组织的肿瘤呈阳性表达，在膀胱、前列腺和胰腺等肿瘤组织中表达较弱。结论 Cyr61在多数肿瘤组织中均过表达，在不同肿瘤组织中表达程度不同，Cyr61可能成为新的肿瘤标志物应用于多种肿瘤的诊断。 【关键词】 Cyr61；组织阵列分析肿瘤, 各部位免疫组织化学Cyr61 expression in several human tumor tissues Li Zhiqiang, Si Weike, Ding Wei, Li Jun, Pan Jing (Department of Clinical Hematology, College of Medical Laboratory, Third Military Medical University, Chongqing, 400038, China) 【Abstract】 Objective To observe and analyze the expression of Cyr61, a secreted, cysteine-rich, heparin-binding protein encoded by a growth factor-inducible immediate-early gene, in a variety of human tumors, in order to explore its role as a new marker for tumor diagnosis. Methods HE and immunohistochemisty staining were used to detect the expression of Cyr61 in several organ tumor tissue array and 30 human tumors samples. Results The expression of Cyr61 was found in human tumor tissue array and in human tumor samples with positive straining in 77% (38/48) and 93% (38/48), respectively. Strong positive staining was found in the tumors from the brain, esophagus and thyroid. Positive staining was seen in the tumors from the stomach, liver, colon, rectum, lungs, kedneys, uterine, skin, ovary and breasts. Negative staining was defined in the tumors from the prostate, bladder and pancreas. Conclusion Overexpression of Cyr61 is seen in many human tumors and the expression is different in different types of tumors. Cyr61 is probably used to diagnose the tumor as a new marker. 【Key words】 Cyr61; tissue microarray; neoplasms by site; immunohistochemistry; human Supported by the General Program of the National Natural Science Foundation of China . Corresponding author: Si Weike, 86-23-687523