当归多糖对大鼠肝性脑病的预防作用.docVIP

当归多糖对大鼠肝性脑病的预防作用 卿 轶,董 志 (重庆医科大学药学院药理学教研室,重庆,400016) 摘 要：目的 观察当归多糖(angelica sinensis polysaccharides, ASP)对四氯化碳性肝硬化大鼠肝性脑病发生的预防作用,并探讨其机制。方法 400 ml/L四氯化碳皮下注射造模,分别给与蒸馏水、乳果糖(50 g:100 ml)、ASP10 g/L、ASP20 g/L、ASP40 g/L溶液10 ml?kg-1?d-1灌胃,连续14 d后用内毒素（3 mg/kg）诱导大鼠昏迷，观察注射内毒素后12 h内各组大鼠昏迷发生时间和数量，测定各组大鼠的血氨、血清谷丙转氨酶（GPT）、谷草转氨酶（GOT）浓度，观察脑细胞凋亡情况和星形胶质细胞数量。结果 各组大鼠血氨、GPT、GOT水平、脑细胞凋亡数和昏迷率，阴性组最高，ASP40 g/L组的血氨、GOT水平和昏迷率均低于阳性药物组，ASP3个剂量组的脑细胞凋亡数均低于阳性药物组,而星形胶质细胞数阴性组最低，且ASP3个剂量组均高于阳性药物组。 结论 ASP可保护脑功能，减缓肝性脑病的发生，作用优于阳性药物乳果糖，其机制可能与降低血氨，改善肝功能，保存星形胶质细胞和神经元的活性有关。 关键词：当归多糖；肝性脑病；凋亡；胶质纤维酸性蛋白 中图法分类号：R243 文献标识码：A Angelica sinensis polysaccharides prevents rat from hepatic encephalopathy QING Yi, DONG Zhi (Biochemical and Molecular Pharmacology Laboratory, Chongqing Medical University, Chongqing 400016, China) Abstract: Objective To investigate to the role of angelica sinensis polysaccharides (ASP) against hepatic encephalopathy and discuss the probable mechanism. Methods Sprague-Dawley rats were established into carbon tetrachloride (CCl4)-induced liver cirrhosis, then treated with distilled water (negative control), lactulose (positive control), ASP solution (10, 20 and 40 g/L respectively, intragastrically at dose of 10 ml?kg-1?d-1) for 14 days. On 15th day, all the survival rats were intraperitoneally injected with endotoxin (3 mg/kg) once, then in the following 12 h, the rats that fall into coma were counted and the time period of coma was recorded. The blood plasma ammonia concentration, serum glutamate-pyruvate transaminase (GPT) and glutamic oxaloacetic transaminase (GOT) concentration were determined. The apoptotic cerebral cells were dyed in situ by TUNEL. Glial fibrillary acidic protein (GFAP) in astrocytes was determined by immunohistochemical method. Then we counted the apoptotic cerebral cells and GFAP positive cells under light microscope. Results The survival rats in distilled water group were highest in the concentration of ammonia, GPT and GOT, the amo