基于第二代测序技术的香菇基因组分析和子实体发育相关的转录因子的初步.ppt

6 试验的可行性及创新点 可行性 已获得W26和L205香菇单核菌株的Short-read测序结果； 真菌基因组的测序与分析手段现在已经比较成熟， Laccaria bicolor、Coprinopsis cinerea、Schizophyllum commune、Fibroporia radiculosa等担子菌模式物种基因组已发表，给以真菌基因组的研究者们很多借鉴； 对一些生物信息学的软件能熟练应用，比如：ncbi-blast+、Velvet、RepeatMasker、GeneMark_ES、Interproscan和Blast2go等软件； 有专门服务器用于进行生物信息学程序的运行，对大部分生物信息学软件所依赖的Linux系统以及常用的Perl编程语言能熟练掌握。 创新点 香菇作为一种重要的应用型（食用）真菌，其基因组的还未公布和发表；对其基因组的研究依旧是一个重大的挑战；本课题以短片段文库（100-bp paired-end测序，300-bp插入片段文库）的测序结果，来组装出基因组，获得尽可能多的基因组信息。 在基因组内搜寻与香菇生长发育香菇的转录因子，搜寻转录因子作用的目的基因，并加以验证，能在一定程度上阐明香菇的子实体发育机理。 7 参考文献 1. Mach, C.M., et al., Evaluation of active hexose correlated compound hepatic metabolism and potential for drug interactions with chemotherapy agents. J Soc Integr Oncol, 2008. 6(3): p. 105-9. 2. Hyodo, I., et al., Nationwide survey on complementary and alternative medicine in cancer patients in Japan. J Clin Oncol, 2005. 23(12): p. 2645-54. 3. Terakawa, N., et al., Immunological effect of active hexose correlated compound (AHCC) in healthy volunteers: a double-blind, placebo-controlled trial. Nutr Cancer, 2008. 60(5): p. 643-51. 4. Shah, S.K., et al., An Evidence-Based Review of a Lentinula edodes Mushroom Extract as Complementary Therapy in the Surgical Oncology Patient. Journal of Parenteral and Enteral Nutrition, 2011. 35(4): p. 449-458. 5. Zhang, Y., et al., Advances in lentinan: Isolation, structure, chain conformation and bioactivities. Food Hydrocolloids, 2011. 25(2): p. 196-206. 6. Yandell, M. and D. Ence, A beginners guide to eukaryotic genome annotation. Nature Reviews Genetics, 2012. 13(5): p. 329-342. 7. Haas, B.J., et al., Approaches to Fungal Genome Annotation. Mycology, 2011. 2(3): p. 118-141. 8. Miller, J.R., S. Koren, and G. Sutton, Assembly algorithms for next-generation sequencing data. Genomics, 2010. 95(6): p. 315-327. 9. Ter-Hovhannisyan, V., et al., Gene prediction in novel fungal genomes using an ab initio algorithm with unsupervised training. Genome Research, 2008. 18(12): p. 1979-1990. 10. Conesa, A., et al.,