丙酮丁醇梭菌发酵蜜生产丁醇的发酵条件优化.pdf

Guangxi Sciences 2011, 18( 3) :278~ 282,288 Butanol Yield from Sugarcane Molasses by Clostridium acetobuty licum and Optimization of Its Fermentation Conditions 米慧芝, 杨登峰, 关 妮, 苏海锋, 黄日波* MI Huizhi,Y NG Dengfeng, GU N Ni, SU Haifeng, HU NG Ribo (, , , 530007) (State Key Laboratory of Nonfood Biomass and EnzymeT echnology,National Engineering Re- search Center for Nonfood Biorefinery, Guangxi cademy of Sciences,Guangxi Key Laboratory of Biorefinery,Nanning,Guangxi,530007,China) : , ( NH ) HPO 0. 3%,H NCONH 0. 1%Na HPO 4 2 4 2 2 2 4 0.5%,KH PO 0.5%, L 0. 025%, 2 4 09%, NaH PO 0. 1%, 11.0536g/L, 2358% 2 4 : : TQ9206 : : 1005-9164(2011)03-0278-05 Abstract:Using singlefador experiments combined with orthogonal design qitimization the fer- mentation conditions, which the clostridium aletobutylicum ferment sugercace molasses to pro- ducte batand and verification test. T he obtained optimal combination of nutrients is 0. 3% (NH4 ) 2 HPO4 , 0. 1% H2NCONH2 , 0. 5% Na2HPO4 , 0. 5% KH2PO4 , 0. 025% Lcysteine hydrochloride monohydrate, 0. 9% tryptone, and 0. 1% NaH2PO4 . The results show that the butanol yield in this optimized fermentation reaches 110536g/ L, which increased about 2358% compared to that obtained from the original strain. T his method has the potential to improve the multichannel utilization of sugarcane molasses. Key words: Clostridium acetobuty licum, sugarcane molasses, butanol, orthogonal test ,: , ,; , , ; , ; , [ 1, 2] , , ,, [3] , , 2.5%~ 3% [4]