血流动力学改变导的兔基底动脉顶端动脉瘤模型.doc

血流动力学改变诱导的兔基底动脉顶端动脉瘤模型 姚鹏飞 杨鹏飞 张照龙 黄清海 刘建民 第二军医大学长海医院神经外科 上海 200433 基金项目：上海市科委科委动物实验专项12140901801）；科委基础研究重点项目 11JC1415800）；科技启明星计划A类11QA1408400）；国家自然基 姚鹏飞 第二军医大学博士研究生，现工作单位：兰州军区兰州总医院 通信作者： [摘要] 目的：颅内动脉瘤模型绝大多数为小动物，不利于血流动力学及血管内治疗研究。本研究通过结扎新西兰大白兔双侧颈总动脉，诱导基底动脉顶端动脉瘤生成。方法：新西兰大白兔26只，分别游离双侧颈总动脉，不行结扎或结扎双侧颈总动脉以增加基底动脉血流。分别于结扎前（0d）、术后1d、1w、4w经颅多普勒测量基底动脉血流速度。术后4w行脑血管造影了解基底动脉变化；术后1w、4w取基底动脉观察动脉形态变化及分叉部行组织学检查。结果：假手术组动物全部成活，实验组术后存活率80%（16/20）。实验组术后1d，基底动脉血流速度直线升高，高于术前231%；术后1w仍在持续升高；术后4w血流速度处于稳定状态。实验组术后1w，基底动脉增粗，明显迂曲；而假手术组无明显变化。实验组基底动脉分叉部中膜厚度变薄；内弹力层部分消失、断裂或变薄。结论：新西兰大白兔双侧颈总动脉结扎术后，基底动脉分叉部血流增加导致基底动脉形态发生改变，并且诱导基底动脉顶端呈现新生动脉瘤样改变。 [关键词]：颅内动脉瘤；模型；血流动力学；基底动脉 Basilar terminus aneurysm model of rabbit induced by hemodynamic changes Yao Peng-fei Yang Peng-fei Zhang Zhao-long Huang Qing-hai Liu Jian-min Department of Neurosurgery, Changhai Hospital, Second Military Medical University, Shanghai 200433, China Objective Most of intracranial aneurysm models were small animals, which were not conducive to study on hemodynamics and endovascular therapy. This study induced the aneurysm formation at the basilar terminus by ligation of the bilateral common carotid artery of the New Zealand white rabbit. Methods Twenty six adult New Zealand white rabbits were subjected to sham, or bilateral common carotid artery ligation to produce basilar artery flow increase. Basilar artery flow velocity was monitored by transcranial Doppler, before the operation, and 1d, 1w, 4 weeks after surgery. Cerebral angiography was performed 4 weeks after surgery. The basilar arterys were seperated and compared morpholodical changes. Bifurcation tissues were harvested at 4 weeks and examined histologically. Results All animals survived in the sham group, and 80% ( 16 /20 ) survived in the experimental group. Subsequent to bilateral common carotid artery ligation, basilar artery flow velocity increased straightly, by 231% compared with the preoperation. The flow velocity kept on increasing 1 w after th