法舒地尔对脂多糖导的小鼠急性肺损伤的保护作用.doc

法舒地尔对脂多糖诱导的小鼠急性肺损伤的保护作用 赵新云 孔辉 王晶晶 闫晓培 刘汶睿 曾晓宁 解卫平 （南京医科大学第一附属医院呼吸科 南京 210029）【摘要】目的探讨R激酶抑制剂法舒地尔fasudil）对脂多糖（lipopolysaccharideLPS）所致小鼠急性肺损伤（acute lung injuryALI）的保护作用及机制。方法C57BL/6小鼠随机分为对照（ontrol）组、LPS组、LPS+asudil（10 mg/kg）组、LPS+地塞米松（dexamethasone，Dex5 mg/kg）组。造模后6 h处死小鼠收集血清、支气管肺泡灌洗液（BALF）、肺组织，血清中TNF-α、IL-1β、IL-10水平HE染色观察各组肺组织病理改变，肺。结果asudil可改善动物生存率延长生存时间；，降低MPO，血清中促炎细胞因子IL-1β、TNF-α，抗炎细胞因子IL-10表达。结论asudil可有效LPS所致的小鼠，其作用。 关键词急性肺损伤脂多糖法舒地尔Fasudil attenuates lipopolysaccharide-induced acute lung injury in mice Zhao Xingyun, Kong Hui, Wang Jingjing, Yan Xiaopei, Liu Wenrui, Zeng Xiaoning, Xie Weiping (Department of Respiratory Medicine, the First Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu 210029,China) Corresponding author: Xie Weiping, Email: wpxie@njmu.edu.cn 【Abstract】Object: To investigate the protective effects of fasudil, a Rho kinase, on the inflammation of lipopolysaccharide (LPS)-induced mice acute lung injury (ALI) secondary to sepsis and it’s probably mechanism in mice. Methods: C57BL/6 mice were randomly assigned to Control group; LPS group; LPS+fasudil (10mg/kg) group; LPS+Dexamethasone (Dex, 5mg/kg) group. Mortality rates of different time points of each group was assessed after the model established. Mice were sacrificed at 6h after LPS injection. Serum, bronchoalveolar lavage fluid 基金项目：国家自然科学基金；江苏省人事厅六大人才高峰（2008074）；江苏省科技厅科技支撑计划（BE2011801）；江苏省呼吸病临床医学研究中心（BL2012012） 通讯作者(Corresponding auther)：Email：wpxie@njmu.edu.cn (BALF) and lung tissues were collected. Elisa was performed to analyze the level of TNF-α、IL-1β and IL-10 in the serumstain with hematoxylin and eosin; test the wet-to-dry (W/D) weight; measure the content of myeloperoxidase (MPO) content in lung tissue. Results: Fasudil significantly improved the mortality rates and prolonged the survival time of mice, reliefed of inflammatory injury and edema of lung tissue, decreased MPO content. Moreover, fasudil down regulated the