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手术部位标本采集doc(Surgical site specimen collection doc)
手术部位标本采集doc(Surgical site specimen collection doc)
If we do not attach importance to etiological diagnosis, it will lead to the use of antibiotics and the emergence of drug-resistant bacteria. It can lead to a large number of hospital infection cases and the outbreak of hospital infection outbreak crisis.
I. General principles
1. samples are collected only before the use of antimicrobial agents, and only when symptoms of clinical infection or deterioration of the wound or long term failure can occur.
2. skin or mucous membrane surface cleaning.
(1) closed wound and puncture specimens: disinfection methods, disinfection of skin with blood culture specimens (see standard procedures for collection and delivery of blood cultures).
(2) open wound: sterile saline, adequate irrigation of wound site. No disinfectant.
3. fresh infected tissues were collected to avoid superficial tissue debris.
4. if swabs or biopsy specimens can be collected, swab specimens should be avoided.
Two, containers
1. larger specimens: a sterile plastic container with threaded threads containing a small amount of physiological saline.
2. specimens smaller or swab specimens: MW E TRANSWAB transport medium, suitable for aerobic and anaerobic bacteria.
Three 、 collection method
(I) closed abscess;
1. syringe to extract pus.
2. if you can not get pus, you should first subcutaneous injection of a small amount of sterile physiological saline, again puncture suction pus; if the pus is too much, should first cut and drainage, in the basement or abscess wall specimens. The amount of pus should be greater than 1ml.
3. exclude the gas inside the syringe and needle, and use a sterile rubber stopper to close the needle for examination; or directly into the blood culture bottle. Suspected anaerobic bacteria should rapidly pour pus into the anaerobic blood culture bottle.
(two) tissue and biopsy specimens
1. collect large enough tissue, the volume of 1mm3 is appropriate to avoid collecting in the necrotic area.
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