(文献四)Endophytic fungi from the medicinal plant Lippia sidoides.pdf

Symbiosis (2011) 53:89–95 DOI 10.1007/s13199-011-0113-7 Endophytic fungi from the medicinal plant Lippia sidoides Cham. and their antimicrobial activity Virginia Medeiros de Siqueira Raphael Conti Janete Magali de Araújo Cristina Maria Souza-Motta Received: 15 December 2010 / Accepted: 7 March 2011 /Published online: 6 April 2011 # Springer Science+Business Media B.V. 2011 Abstract Endophytic fungi were isolated from healthy Keywords Endophytic fungi .Lippia sidoides . tissues of Lippia sidoides Cham. (Verbenaceae), a medic- Antimicrobial activity inal plant used as an antiseptic in the northeast of Brazil. From 480 fragments of leaves (240) and stems (240), a total of 203 endophytic fungi were isolated, representing 14 1 Introduction species belonging to the groups Ascomycota , Coelomycetes and Hyphomycetes . Endophytic colonization was greater in The establishment of higher plants in their respective leaves (50.4%) than in stems (35.4%). Colletotrichum habitats involves a capacity to interact with different gloeosporiodes had the maximum colonization frequency organisms. Endophytes colonize inter and/or intracellularly (12.3%), followed by Alternaria alternata (7.08%), the healthy living tissue of host plants, typically without Guignardia bidwelli (6.87%) and Phomopsis archeri causing any visible symptoms of disease (Petrini 1991; (5.41%). Some species showed specificity for the host Azevedo et al. 2000; Saikkonen et al. 2004). The tissue: Curvularia pallescens, Dreschlera dematioidea, G. relationship between an endophyte and its host is complex bidwellii, Microascus desmosporum, Peacilomyces variotti, (Owen and Hundley 2004). Fungi are able