2型糖尿病对大鼠心肌缺血再灌注损伤救援激酶信号通路影响.doc

2型糖尿病对大鼠心肌缺血再灌注损伤救援激酶信号通路影响.doc

  1. 1、本文档共11页,可阅读全部内容。
  2. 2、有哪些信誉好的足球投注网站(book118)网站文档一经付费(服务费),不意味着购买了该文档的版权,仅供个人/单位学习、研究之用,不得用于商业用途,未经授权,严禁复制、发行、汇编、翻译或者网络传播等,侵权必究。
  3. 3、本站所有内容均由合作方或网友上传,本站不对文档的完整性、权威性及其观点立场正确性做任何保证或承诺!文档内容仅供研究参考,付费前请自行鉴别。如您付费,意味着您自己接受本站规则且自行承担风险,本站不退款、不进行额外附加服务;查看《如何避免下载的几个坑》。如果您已付费下载过本站文档,您可以点击 这里二次下载
  4. 4、如文档侵犯商业秘密、侵犯著作权、侵犯人身权等,请点击“版权申诉”(推荐),也可以打举报电话:400-050-0827(电话支持时间:9:00-18:30)。
查看更多
2型糖尿病对大鼠心肌缺血再灌注损伤救援激酶信号通路影响

2型糖尿病对大鼠心肌缺血再灌注损伤救援激酶信号通路影响  【摘要】 目的 探讨2型糖尿病(T2DM)对心肌缺血后适应(ischemic postconditioning,IPO )减轻心肌缺血再灌注损伤作用的影响及可能机制。方法 高脂饮食联合STZ诱导制成T2DM大鼠模型,将60只雄性Wistar大鼠随机分为正常大鼠缺血再灌注组(A组)、正常大鼠缺血后适应组(B组)、糖尿病大鼠后适应组(C组)。3组均采用离体大鼠心脏Langendorff灌流方法,全心停灌30 min,复灌60 min,制成心肌缺血再灌注模型。B、C组在再灌注开始前先给予再灌注10 s,全心停灌10 s,共6次循环的IPO。免疫组织化学染色及Western印迹法测定心肌磷酸化Akt,磷酸化糖原合成酶激酶(GSK3β)的表达。结果 正常离体大鼠心肌IPO干预后磷酸化Akt及GSK3β的表达增强;而对T2DM大鼠给予IPO处理后磷酸化Akt及GSK3β的表达无增强,去磷酸化GSK3β表达增强。结论 IPO对正常大鼠离体心脏缺血再灌注损伤有明确的保护作用,而对T2DM大鼠心肌缺血再灌注损伤无保护作用;其机制可能与糖尿病状态下影响再灌注损伤救援激酶信号通路,导致GSK3β活性(去磷酸化水平)增高有关。 【关键词】 缺血后适应;糖尿病;缺血再灌注损伤;再灌注损伤救援激酶信号通路 【Abstract】 Objective To elucidate the effects of ischemic postconditioning(IPO) on ischemia/reperfusion(I/R) cardiac and the role of reperfusion injury salvage kinase pathway in type 2 diabetic rats . Methods The type 2 diabetic rats were induced by the intravenous injection of streptozotocin and high caloric diet. Sixty Wistar rats were randomly devided into I/R in nomal rats(A), IPO in normal rats(B), IPO in diabetic rats(C) groups. Rats were given for Langendorff isolated heart perfusion with 30 min of globe ischemia and 60 min of reperfusion, then the models of group A were made. But the rat hearts of group B and C were subjected to 6 cycles of 10 min of globe ischemia and 10 min of reperfusion as IPO during the early minutes of reperfusion. Phosphorylation of akt and gsk3β were analyzed by Western blot and immunohistochemical staining. Results The phosphoakt and phosphogsk3β expressions were increased markedly in B group. But compared those in A group there was no difference in C group. The phosphoakt and phosphogsk3β expressions in C group were more less than those in B group. Conclusions IPO may significantly protect myocardium in isolated normal rat hearts . But in diabetic rats, the protection of IPO has no effect, the mechanism of this phenominon maybe related to gsk3β in the condition of diabetic.   【Key words】 Ischemic postconditioning; Diabetic ; Ischemic /reperfusion injur

文档评论(0)

linsspace + 关注
实名认证
内容提供者

该用户很懒,什么也没介绍

1亿VIP精品文档

相关文档