BFGF及TGF-β1及260例乙肝患者肝脏纤维化及炎症关系探究.doc

BFGF及TGF-β1及260例乙肝患者肝脏纤维化及炎症关系探究 　作者：康海燕，董江龙，叶立红，王建彬，刘莲，殷树英 【关键词】 乙型肝炎 碱性成纤维细胞生长因子 转化生长因子β1 肝纤维化 免疫组化 Abstract:　　Objective To observe the immunolocation and expression of basic fibroblast growth factor (BFGF) and transforming growth factor β1(TGF-β1) in different stage on liver fibrosis induced by CHB (chronic hepatitis B), and to discuss their effects in the development of liver fibrosis and the correlation with hepatitis and fibrosis markers.Methods BFGF and TGF-β1 in the liver of 260 patients with CHB were evaluated with immunohistochemistry and quantitive measurements. The relationship between BFGF/TGF-β1 and staging of hepatic fibrosis/grading of inflammation was analyzed.Results Expression of BFGF and TGF-β1 increased in line with the elevation of fibrotic staging. The levels of BFGF and TGF-β1 in groups of S1-S4 were higher than those in control group S0. The differences were statistically significant (Plt;0.05). And there were significant differences between every two groups (Plt;0.05). But in inflammation grading, the surface density in groups G1-G4 were all higher than that in control group G0 (Plt;0.05). But there were no significant differences between every two groups (Plt;0.05). There is close positive correlation between BFGF and TGF-β1 in hepatic tissue, the coefficient was 0.895 (Plt;0.05).Conclusion There is a close positive correlation between BFGF/TGF-β1 and hepatic fibrosis, and no correlation with liver inflammation. BFGF and TGF-β1 can be used as the important indicators of the severity of hepatic fibrosis, and furthermore, the understanding of them may provide theoretica basis of anti-fibrosis therapy by antagonists of BFGF and TGF-β1. Keywords:hepatitis B; basic fibroblast growth factor; transforming growth factor β1; hepatic fibrosis; immunohistochemistry 　　肝星状细胞(HSC)激活分泌大量细胞外基质(ECM)沉积在肝内引起肝纤维化[1]。碱性成纤维细胞生长因子(BFGF)及转化生长因子β1(TGF-β1)可促进ECM合成，导致肝纤维化[2]。本实验以260例慢性乙型肝炎患者肝组织BFGF、TGF-β1为研究对象，观察其表达和定位特点，进而比较二者与肝脏纤维化及炎症的关系，现将结果报道如下。 1 资料与方法 　　1.1