c-myc、c-fos在良性胆道狭窄瘢痕组织中表达及意义.doc

c-myc、c-fos在良性胆道狭窄瘢痕组织中表达及意义 　【摘要】 目的 探讨原癌基因c-myc、c-fos与良性胆道狭窄形成的相关性。进一步阐明良性胆道狭窄中胆管瘢痕的发生机制。方法 应用免疫组化SP法，检测c-myc、c-fos蛋白在肝内、外胆管瘢痕组织中的表达和分布。对其阳性表达结果进行比较分析。结果 肝内、外胆管瘢痕组织的成纤维细胞、胆管上皮细胞及血管内皮细胞中c-myc、c-fos呈强阳性表达，而正常胆管组织中缺乏表达。结论 肝内、外胆管瘢痕组织中c-myc和c-fos癌基因受激活，可能参与了成纤维细胞的分化增殖、胶原合成与降解以及对细胞因子的调控，并导致瘢痕增生。原癌基因c-myc、c-fos与良性胆道狭窄瘢痕形成密切相关。c-myc与c-fos蛋白两者的表达成正相关，两者具有协同性。 【关键词】 原癌基因c-myc 原癌基因c-fos 良性胆管狭窄 胆管瘢痕 Expression of c-myc、c-fos in scartissue of benign biliary stricture and correlation investigation LU Hui，ZHANG Xiao-wen,LIU Wen-yong.Department of Surgery,Fengcheng Hospital,Shanghai 201411,China 【Abstract】 Objective To observe the expression and distribution of c-myc and c-fos in biliary scar tissue, to investigate the correlation between expression of key pro-oncogenes and benign biliary stricture. Methods Immunohistochemical technique was used to detect the expression and distribution of c-myc、c-fos proteins on extrahepatic bile duct scar tissue、intrahepatic bile duct scar tissue and normal bile duct tissue. Results c-myc and c-fos expression on the nucleus of fibroblast、epithelial cell and endothelial cell of biliary scar tissues were significantly higher than normal biliary tissue. And there was obviously difference between scar tissue and normal tissue.Conclusion c-myc and c-fos oncogenes are activated on biliary scar tissues,which may contribute to proliferation and differentiation of fibroblasts、synthesis and degradation of collagen and regulation of cytokines and induce abnormal scarring.There was close corralation between c-myc、c-fos proto-oncogenes and benign biliary stricture scar tissue.there were positive correlations and co-operativity between c-myc and c-fos expression. 【Key words】 proto-oncogenes c-myc；proto-oncogenes c-fos；benign biliary stricture；bile duct scar 胆道狭窄一直是胆道外科领域中病变复杂处理困难的课题。 近来一些研究表明， 部分原癌基因在组织再生等过程中常被激活， 可能通过调控某些细胞因子来控制病理性瘢痕增生。 但对于在胆道瘢痕的形成过程中,原癌基因是否参与调控及表达尚不清楚。 本研究探讨了肝内、 外胆管瘢痕组织中c-myc和c-fos蛋白的表达及c-myc、 c-fos的表达与胆管瘢痕形成的相关性。 试从分子水平来了解良性胆道狭窄形成机制