KGF在乳腺癌表达及临床意义.doc

KGF在乳腺癌表达及临床意义 　 作者：王秀梅 黎莉 郝静 张芳 王妍 刘友德 【摘要】目的：探讨KGF蛋白在人乳腺癌的表达与意义，与临床病理因素及生物学指标的相关性。方法：采用免疫组化方法检测KGF在63例乳腺癌组织中的表达，并与正常乳腺组织对照。结果：63例乳腺癌组织中34例肿瘤细胞表达KGF（54.0%），正常乳腺上皮细胞未表达（P＜0.01），在乳腺癌间质细胞和正常乳腺组织间质细胞表达率分别为34.9%和16.7%（P＞0.05）。其在肿瘤细胞中的表达与低分化相关，与ER、PR呈负相关，与Ki67呈正相关，与Ki67的协同表达与淋巴结转移正相关。结论：KGF在乳腺癌肿瘤细胞的表达可能促进乳腺癌的发生发展，并且与低分化、预后不良有关。 【关键词】角质细胞生长因子#12539;乳腺肿瘤#12539;病理学，临床 Expression of KGF in human breast cancer and its clinical significance 【ABSTRACT】Objective:To investigate expression of keratinocyte growth factor(KGF)and its clinical significance in human breast cancer and their correlations with clinicopathological features and biomarkers.Methods:The expression of KGF in 63 cases of breast cancer and 30 cases of normal breast tissue were detected by immunohistochemistry.Results:Of the 63 cases of breast cancer,KGF was expressed in tumor cells in 34 cases(54.0%).While it was not detected in epithelial cells in normal breast tissue.The difference between them had remarkably statistical significance.The expression rate of KGF in mesenchymal cells in breast cancer and normal breast tissue was 34.9% and 16.7%.The difference between them was not remarkable.The expression of KGF in tumor cells had correlation with low differentiation degree,had a negtive correlation with ER and PR and a positive correlation with Ki67.The coexpression of KGF and Ki67 had a positive correlation with lymph node metastasis.Conclusion:The expression of KGF in tumor cells was possibly involved in the development of breast cancer,and had correlations with low differentiation degree and bad prognosis. 【KEY WORDS】Keratinocyte growth factor#12539;Mammary neoplasms#12539;Pathology,clinical 近年来发现，角质细胞生长因子（keratinocyte growth factor,KGF）与癌症如肺癌［1］、乳腺癌［2，3］、卵巢癌［4］、前列腺癌［5，6］、结直肠癌［7］、宫颈癌［8］的发生发展密切相关。已证实［2］重组人KGF以剂量依赖模式明显地刺激正常乳腺和乳腺癌上皮细胞的增生，鼠成纤维细胞来源的KGF可促进ER（+）乳腺癌细胞（MCF7和T47D）增生和迁移［3］。本实验采用免疫组化的方法检测63例乳腺癌组织中KGF的表达以及其表达与ER、PR、CerbB2、Ki67表达的相关性，结合临床病理因素，分析其临床意义。 1 资料与方法 1.1 标本来源 山东大学齐鲁医院经病理检查确诊的乳腺癌63例，均为女性。年龄29