Survivin及p27Kip1蛋白在结肠癌中表达及临床意义.doc

Survivin及p27Kip1蛋白在结肠癌中表达及临床意义 　 作者：曹洪华 梁国林 梁君林 陈利生 高枫 【摘要】 目的 探讨Survivin和p27Kip1蛋白在结肠癌中的表达及其相互关系。方法 应用免疫组织化学方法检测83例结肠癌标本和10例正常结肠黏膜组织中Survivin、p27Kip1的表达。分析Survivin、p27Kip1的表达与临床病理参数的关系。结果 Survivin在结肠癌组织中阳性表达率83.1％（69/83）明显高于在正常结肠黏膜组织中的0.0%（0/10）(Plt;0.01)。结肠癌中Survivin的表达与临床病理参数无关（Pgt;0.05）。p27Kip1在结肠癌组织中阳性表达率41.0%（34/83）明显低于在正常结肠黏膜组织中的90%（9/10）（Plt;0.01）。远处转移组p27Kip1阳性率(16.7%，3/18)明显低于远处无转移组(47.7%，31/65)(Plt;0.05)。结肠癌p27Kip1表达与组织分化、肠壁浸润深度、Dukes分期有关（Plt;0.05）。结肠癌组织中Survivin表达与p27Kip1表达未见明显相关性（Pgt;0.05）。结论 Survivin和p27Kip1蛋白在结肠癌组织中存在异常表达，提示两者在结肠癌的发生发展中起着重要的作用。结肠癌中Survivin的表达与p27Kip1蛋白的表达无相关性。 【关键词】 结肠癌；免疫组织化学；Survivin；p27Kip1 [Abstract] Objective To investigate the expression of Survivin and its correlation with expression of p27Kip1 protein in colon carcinoma. Methods Expression of Survivin and p27Kip1 protein was investigated by immunohistochemistry in 10 cases of normal colon tissues and 83 cases of colon cancer tissues. The relationship between Survivin expression and clinicopathological parameters as well as p27Kip1 expression was analyzed. Results The positive expression rate of Survivin was significantly higher in colon cancer tissue (83.1%, 69/83) than in normal colon tissue (0.0%, 0/10) (Plt;0.01). Expressions of Survivin were not related to the clinicopathological parameters (Pgt;0.05). The positive expression rate of p27Kip1 was significantly lower in colon cancer tissue (41.0%, 34/83) than in normal colon tissue (90.0%, 9/10) (Plt;0.01). The positive expression rate of p27Kip1 in colon cancer was significantly lower in distant metastasis group (16.7%, 3/18) than in nondistant metastasis group (47.7%, 31/65) (Plt;0.05). The expression of p27Kip1 was significantly correlated with histological differentiation, invasion depth and Dukes stages (Plt;0.05). There was not correlation between the expression of Survivin and p27Kip1 (r=0.096，Pgt;0.05).Conclusion The abnormal expression of Survivin and p27Kip1 protein indicates that Survivin and p27Kip1 may play a