Syk基因表达及大肠癌关系探究.doc

Syk基因表达及大肠癌关系探究 　 作者：万伟 吴小鹏 王建平 刘长兴 【摘要】目的：探索Syk基因的表达与大肠癌生成及转移的关系。方法：应用RTPCR技术检测40例大肠癌组织及癌旁正常组织中Syk基因的表达，通过正常组织与癌组织、有无淋巴结转移、浸润深度、临床分期、病理分级进行比较，观察Syk基因的表达与大肠癌生成及转移的关系。结果：40例大肠组织都有Syk基因的表达，而40例大肠癌组织中只有13例表达(32.5%)（χ2=40.755,P＜0.05）；28例有淋巴结转移的大肠癌组织，4例Syk基因表达（14.3%），12例无淋巴结转移，有9例表达（75.0%）（χ2=14.155,P＜0.05）。Syk基因表达与淋巴转移、浸润深度、临床分期有关，与年龄、肿瘤分化等级、大小无关。结论：Syk基因表达缺失与大肠癌生成及转移、浸润深度有关。 【关键词】大肠肿癌#12539;基因，Syk#12539;病理学，临床 Research on the expression of the Syk gene in human colon Carcinoma 【ABSTRACT】Objective:To evaluate the effects of the Syk gene expression in human colonic carcinoma on tumor growth and metastasis.Methods:The expression of Syk gene in the 40 fresh freezed tissues of colonic carcinoma patients(tumor tissues,adjacent normal tissues) was detected by RTPCR technique.Results:All normal colonic tissues were detected the expression of the Syk gene.Compared to normal tissues,27 out of 40 colonic carcinoma did not show any detectable Syk expression.And there were significant difference between the two groups(χ2=40.755,P＜0.05);only 4 colonic carcinoma tissues in 28 patients with lymph node metastasis had the Syk gene expression.9 colonic carcinoma tissues in 12 patients without lymph node metastasis had the Syk gene expression.The Syk gene expression was negatively correlated to lymph node metastasis(χ2=14.155,P＜0.05).Furthermore,the expression of Syk gene was correlated with tumor,lymphnode metastasis ,and infiltration,but no correlation was observed with tumor size differentiation degree,clinical period.Conclusions:Normal colonic tissues have expression of Syk gene.The silencing of Syk gene is correlated to growth and metastasis of colonic carcinoma.The Syk gene expression deletion may play an important role in tumor infiltration and metastasis in colonic cancer. 【KEY WORDS】Colonic neoplasms#12539;Genes,Syk Pathology,clinical Syk（Spleen tyrosine kinase）被认为是一种候选抑癌基因，该基因表达与恶性肿瘤发生有关，本研究探讨结直肠癌中Syk的表达情况及其影响因素。 1 资料与方法 1.1 标本来源 我院手术切除并经病理证实的结直肠癌标本40例，同时取距瘤体5cm外正常结肠组织，置-7