基于特殊形貌CdS纳米颗粒修饰的DNA传感器在DNA-仪器信息网.PDF

第 2 1 卷 　第 8 期 传 感 技 术 学 报 Vol . 2 1 　N o . 8 2008 年 8 月 CHIN ES E J OU RNAL O F S EN SORS AND AC TUA TORS Au g . 2008 CdSModif ied D NA Sensor f or Direct SignalAmpl if ied Sensing of D NA Hybridization X I A Qi ng 1, 2 , L I U J i nh u ai 1 , 2 1. L aboratory of B ionic an d N anotechnology , I ns t it ute of I ntel l i g ent M achi nes , Chi nese A ca demy of S cience , H ef ei 23003 1 , Chi na; 2 . D ep art ment of A utom at ion , Uni vers ity of S cience an d Technology of Chi na , H ef ei 230026 , Chi na Abstract :The Cd S nano sp here s wit h sp ecial walnutlike surf ace are p rep ared by solvot her mal met ho d and charact erized fir st , t hen t he mercap to grouplinked p robe DN A wa s covalently immobilized onto t he Cd S layer and exp o sed to ODN t ar get for hybridization . Sen sitive elect rical readout s coup led wit h Cyclic Volt a mmo gram ( CV ) an d Diff erential Pul se Volt ammo gram (D PV ) t echnique s show significant increa sed re spon se s. The sen sor i s able to robu st ly di scriminat e t he DN A hybridization re sp on se s wit h goo d sen sitivit y an d st abilit y and t he selectivit y of t he sen sor i s t e st ed u sin g a serie s of mat ched and cert ainp oint mi s - 6 1 μ μ mat ched sequence wit h concent ration grad s ran ging f ro m 10 M to 10 mol/ L . The p eak value s are al mo st linear wit h t he minu s lo garit hm of t ar get DN A concent ration s wit h det ection limit 1 p mol/ L