NMR-核磁共振波谱法.pdf

核磁共振波谱法 Nuclear Magnetic Resonance Spectroscopy，NMR Some history of NMR 1946 Principle of solid state NMR (Bloch, Purcell) 1950 Resonance frequency depends on chemical environment (Proctor, Yu) 1953 Overhauser effect 1956 First NMR spectra of protein (Ribonuclease) 1965 Fourier Transform spectroscopy (Ernst) 1973 Imaging tomography (Mansfield) 1985 First protein structure (bovine pancreatic trypsin inhibitor) in solution (Wüthrich) By now: More than 150 protein structures (M 60 000) BPTI Bound water Protein dynamics Functional MRI MRI is used for imaging of all organs in the body. NMR Nobel Prize Winners  1944 Isador Rabi  1952 Felix Bloch Edwin Purcell  1991 Richard Ernst  2002 Kurt Wüthrich  2003 Paul Lauterbur Sir Peter Mansfield The Nobel Prize in Physics 1952 for their development of new methods for nuclear magnetic precision measurements and discoveries in connection therewith Felix Bloch Edward Mills Purcell The Nobel Prize in Chemistry 1991 for his contributions to the development of the methodology of high resolution nuclear magnetic resonance (NMR) spectroscopy Richard R. Ernst The Nobel Prize in Chemistry 2002 for his development of nuclear magnetic resonance spectroscopy for determining the three-dimensional structure of biological macromolecules in solution Kurt Wüthrich The Nobel Prize in Medicine 2003 for their discoveries concerning magnetic resonance imaging Paul C. Lauterbur