流动注射研究糖分.pdf

Journal of Biotechnology 50 (1996) 93-106 Analysis of various sugars by means of immobilized enzyme coupled flow injection analysis B. Weigel”, B Hitzmann”, G. Kretzmer”, K. Schiigerl”,*, A. Huwigb, F. Giffhornb “Institut fir Technische Chemie der Universitiit Hannover, Callinstr. 3, D-30167 Hannover, Germany bLehrstuhl fiir Angewandte Mikrobiologie der Universitiit des Saarlandes, D-66041 Saarbriicken, Germany Received 8 August 1995; revised 1 March 1996; accepted 5 March 1996 Abstract Glucose, maltose, sucrose, lactose, xylose, sorbose, galactose, fructose and gluconolactone were analyzed by means of immobilized pyranose oxidase as well as by the combination of immobilized glucose oxidase with immobilized glycoamylase, invertase, mutarotase, maltase (cc-glucosidase) and glucose isomerase by flow injection analysis (FIA). For the simultaneous analysis of glucose and other sugars three different flow-injection configurations were applied and compared. The average error of prediction of the analyses were better than 3% in model media and better than 6% in yeast extract containing media. Keywords: Sugar analysis; Flow-injection analysis; Immobilized glucose oxidase; Glucoamylase; Invertase; Mutaro- tase; a-Glucosidase; p-Galactosidase; Pyranose oxidase; Glucose isomerase Abbreviations: AAP, 4-aminoantipyrine; ABTS, 2,2’-azino-