第4章intracellular+vesicular+traffic.pdf

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第4章intracellularvesiculartraffic

小结 信号: 信号序列/斑  细胞区室化  蛋白质分选 识别: 受体 跨膜运输: ER, Mt, Chl, Peroxisome 通道: 易位子/ 核孔复合物 门控运输: Nucleus 能量: ATP 跨膜/ 门控运输 第四章 Intracellular vesicular traffic Outline 1 Molecular mechanisms of vesicular traffic 2 Exocytosis and endocytosis pathway Molecular mechanisms of vesicular traffic Morphological and cell-fractionation studies had uncovered a labyrinthine network of intracellular membranes interrelated by the process of vesicular traffic. How are these vesicles created; how do they track to and fuse with a proper target; and how do organelles communicating through a vesicular intermediary maintain their characteristic identity? The Nobel Prize in Physiology or Medicine 2013 for their discoveries of machinery regulating vesicle traffic, a major transport system in our cells. 第三章细胞的物质流 6 Genetic study of the secretory pathway in Saccharomyces cerevisiae —Isolate yeast mutants with temperature sensitive defects in secretion, identify and study molecules involved. —By R. Schekman •Secretory mutants are defined as those strains which fail to export active invertase and acid phosphatase, but continue to synthesize protein under restrictive growth conditio

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