重庆大学本科生(高蝶学号：).docVIP

重庆大学本科学生毕业设计（论文） 五种抗糖尿病中药提取物的PPARs激动活性研究 学 生： 高 蝶 学 号：指导教师： 夏之宁教授 专 业： 药 学 重庆大学化学化工学院 二O一二年六月 Graduation Design (Thesis) of Chongqing University ctivation on PPARs of extracts from five anti-diabetic traditional Chinese medicines Undergraduate: Gao Die Supervisor: Prof.Xia Zhining Major: Pharmacy College of Chemistry and Chemical Engineering Chongqing University June 2012  摘 要 本文通过研究具有抗糖尿病或利尿生津作用的中药金线莲、熟地黄、生地黄、地骨皮以及芦根提取物对PPAR的两个亚型PPARβ和PPARγ的激动作用，探讨其抗糖尿病的作用是否可能是通过PPAR的。 本文先五种中药提取石油醚、乙酸乙酯、70%醇提、水提以及水提醇沉五个部分，然后，以0.1% DMSO及水作为对照，3个复孔，96孔板，针对实验所的Hela细胞进行了细胞毒性实验，了中药进行PPARs研究实验的浓度接着采用Hela细胞筛选模型对中药的进行PPARs激动实验，将报告质粒和PPARγ质粒（或PPARβ质粒）转Hela细胞，得到PPARγ（或PPARβ的Hela细胞，0.1% DMSO及水作为对照0.5 μg/mL的非诺贝特PPARβ组的阳性对照，0.5 μg/mL罗格列酮PPARγ组的阳性对照。最终荧光素酶活性检测，对PPARβ或PPARβ有激动作用的提取物 对于PPARβ有激动作用的包括金线莲（石油醚部分：200μg/mL）、熟地黄（石油醚部分：100μg/mL）及芦根（乙酸乙酯部分：200μg/mL）。五种中药对于PPARγ均有一定激作用，其中以芦根的乙酸乙酯部分的激作用最为显著，激活倍数达7.48倍。 关键词：糖尿病，中药提物，过氧化物酶体增殖物激活受体(PPAR)，天然激动剂  ABSTRACT In the present study, the activities of the crude extracts of traditional Chinese medicines (TCMs) on PPARβ and PPARγ were investigated. The tested TCMs include Ancecotochilus roxburghii, Radix rehmanniae praeparata, Rehmannia glutinosa, Lycii cortex and Rhizomes phragmitis, which have anti- diabetes or diursis nourish effects. Furthermore, the anti-diabetes mechanism of the tested TCMs, which may act on PPARs, is also discussed. Firstly, the five extracts of petroleum ether, ethyl acetate, 70% ethanol, water and water extraction and alcohol precipitation for each TCM were prepared. Then using a 96-well plate, the MTT toxic test was done on different concentrations, while 0.1% DMSO and water were used as control groups. Each sample repeat for three-cells and the range of preferable administration concentrations for the TCM extracts on the PPARs screening model were chosen. At last, transfecting the report plasmid and the report plasmid with PPARγ (or PPARβ) expressed plasmid (screening cell model of PPARγ or