徕卡激光共聚焦原理.pdf

Living up to Life 徕卡激光共聚焦显微镜 的必威体育精装版进展及应用 市场部 徐建平 几个概念 Living up to Life 激光扫描共聚焦显微镜 CLSM——ConfocalLaserScanningMicroscope 徕卡共聚焦系统 LeicaTCS——LeicaTrueConfocalSystem 声光调制滤片 AOTF——Acousto-OpticalTunableFilter 光电倍增管 PMT——PhotomultiplierTube 徕卡共聚焦软件 LASAF——LeicaApplicationSuiteAdvanced Fluorescence 像素 Living up to Life 扫描分辨率 Living up to Life 灰阶 Living up to Life 物镜 Living up to Life 色环含义 放大倍数 物镜等级 数值孔径 5 × 红 10 × 黄 20 × 绿 40 × 蓝 色环 63 × 深蓝 100 × 白 工作距离 Living up to Life 物镜数值孔径 Living up to Life 光学分辨率 Living up to Life resolved Limit of resolution Not resolved R Diagram of diffraction limited image of beads Two very close diffraction Single diffraction Airy disk . . . . limited spots .. limited spot Size of beads 1.22  横向分辨率: Rlateral = NA + NA objective