抗ra33抗体的检测及其在类风湿关节炎中的意义（Detection of anti RA33 antibody and its significance in rheumatoid arthritis）.docVIP

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抗ra33抗体的检测及其在类风湿关节炎中的意义（Detection of anti RA33 antibody and its significance in rheumatoid arthritis）.doc

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抗ra33抗体的检测及其在类风湿关节炎中的意义（Detection of anti RA33 antibody and its significance in rheumatoid arthritis）

抗ra33抗体的检测及其在类风湿关节炎中的意义（Detection of anti RA33 antibody and its significance in rheumatoid arthritis） Detection of anti RA33 antibody and its significance in rheumatoid arthritis 2010-01-13 Liu Jianping, Ceng Qingyu, Zhou Xiuguo, Xiao Zhengyu, Xu Jiezhou, Huang Shaobi To investigate the anti RA33 antibody in rheumatoid arthritis (RA) diagnosis, early diagnosis and prognosis, provide clues for individualized therapy in 1, we use the method of Hassfeld to RA and other 280 cases were detected, the results are as follows. 1 data and methods 1.1 study subjects Patients: 125 patients with RA, 42 males and 83 females, aged 18 to 76 years, with an average age of 46.1 years. Among them, 37 cases were early. No use of slow acting antirheumatic drugs, or 66 patients with less than 1 months (referred to as those who did not use drugs). The diagnosis was consistent with the 1987 American College of Rheumatology (ARA) RA classification criteria. The control group: 155 cases, including 111 cases of various rheumatic diseases, including systemic lupus erythematosus (SLE), 54 cases of ankylosing spondylitis (AS), 47 patients with systemic sclerosis (SSc) in 10 cases, the diagnosis was consistent with the international general diagnostic standard corresponding; another 44 cases of normal control group. 1.2 method 1.2.1 collected serum samples were recorded over the same period in general, joint performance (tender joint count, number of swollen joints, arthritis index), disease severity (Strinbrocker staging), extra articular manifestations, physicians and patients on disease activity evaluation. The joint performance was evaluated by 28 joint scoring method [2], and the X-ray progress of hand, foot and joint was evaluated by the improved Sharp scoring standard. 1.2.2 culture collection is about 2 * 109 HeLa cells, according to Hassfeld extraction method of RA33 antigen, 12% by twelve sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS), and then electrophoretically transf