母种分离技术（Mother seed separation technique）.doc

母种分离技术（Mother seed separation technique） In nature, edible fungi do not exist alone, but with many bacteria, radiation bacteria, fungi and other living together. The so-called separation of bacteria is to separate these living bacteria with edible fungi, and to obtain pure fine strains through culture. Spawn, denominator, seed, and cultivated species. Seed production procedures refer to figure 13. (1) isolation and cultivation of mother species Separation of mother edible fungus, can be divided into spore separation, tissue isolation method and hypha separation etc.. 1. spore isolation Spore separation is the method of culturing mycelia by the germination of sexual spores or asexual spores of edible fungi. This strain has strong viability, but there are differences between individual spores, and the phenomenon of natural differentiation is serious, and the variation is great, so it needs to be tested by fruiting to be applied in production. (L) single spore isolation: the method of obtaining only one spore per time or each test tube and allowing it to germinate into mycelium to obtain pure bacteria. By single spore isolated from the mycelium of mushroom and mushroom, have strong ability, this method can be used to produce pure strains isolated. The production of single spore isolation is less, and the technology is complex. Generally, the method of spore isolation is adopted. (2) multiple spore isolation: a method of inoculating many spores on the same medium, allowing them to germinate and freely mate to obtain pure strains of edible fungi. Specific methods of operation, there are several: A mushroom spore ejection method: individual robust, round shaped flower, no pests, fruiting uniform, high yield, strong adaptability of the mushroom, eight or nine mature cut parts, stipe gauze or cotton, wash water and dry with filter paper surface sterilized several times with sterile water. In the indoor inoculation box or the kind of sterile, mushroom gills down with wire ha