条斑紫菜叶状体dna提取方法的研究-吉林农业大学.pdf

吉林农业大学学报　2007, 29 (6):640 ～642, 646 http ://xuebao.lau . Journal of J ilin Agricultural University E -mail :lndxb @vip . DNA 王　萍, 倪伟镯, 孟学平 (淮海工学院海洋学院, 江苏省海洋生物技术重点建设实验室, 连云港222005) 　:以常用的植物基因组DNA 提取方法—LiCl 法、SDS 法、CTAB 法、高盐脲法和试剂盒法提取条斑紫 叶状体DNA, 对不同提取方法的效果进行了比较分析, 同时探讨了SDS 法的细胞破碎方法和不同样本量提取 条斑紫 DNA 的效果。 对DNA 纯度、DNA 浓度、DNA 得率等分析表明, SDS 法效果较好, 其次是LiCl 法;在使 用SDS 法时, 液氮研磨破碎细胞效果好于其他方法;在小样本量提取DNA 时, SDS 法比LiCl 法效果好。 :条斑紫 ;叶状体;DNA 提取 :Q78;Q949.321　　　:A　　　:1000-5684(2007)06-0640-03 Study on Methods of DNA Extraction from Thallus of Porphyra y ezoensis WA NG Ping, NI Wei-zhuo, MENG Xue-ping (School of Marine Science and Technology , Huaihai Institute of Technology , J iangsu Key Laboratory of Marine Biotechnology , Liany ungang 222005, China ) Abstract:DNA from thallus of Porphyra yezoensis was xtract d by m thod of LiCl, SDS, CTAB, High- salt Ur a and Plant G nomic DNA Isolation Kit, and th ff cts of diff r nt xtraction m thods w r com- par d and analyz d.At th sam tim , th ff cts of smudg c ll m thods and diff rr nt sampl siz s to xtract th g nomic DNA of Porphyra yezoensis w r discuss d.By valuating purification of DNA, con- c ntration of DNA, and DNA yi ld, th r sults indicat d that th ff ct of SDS was b tt r than oth r m thods, and th n was LiCl.Wh n SDS was us d, th smudg c ll by skiv with liquid nitrog n had th b st ff ct among th s m thods.SDS to xtract DNA was b tt r than LiCl in small sampl . Key words:Porphyra yezoensis;thallus;DNA xtraction 　　(Porphyra spp.)(Rhodophyta) , , 、 (Protoflorid ophyc a )(Ban- , giac a ), , 、 , , , 。, , , , 。