电子断层成像术.pdf

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电子断层成像术.pdf

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电子断层成像术

7. Tomography and Subtomogram Averaging Tanmay Bharat EM course 2014 What is tomography? Steven and Aebi, Trends Cell Biol, 2003 What is tomography? tilt series is used to obtain a tomogram Tilt series Tomogram The principle of 3D reconstruction analogous to single particle analysis What is tomography? tilt series is used to obtain a tomogram Of course, in tomography we have very good prior information of orientations!! *repeated from lecture 6 by Sjors Scheres What is tomography? Overall workflow Lucic and Baumeister, JCB, 2013 What is tomography? Overall workflow OK, this is great! To what can we apply tomography? Lucic and Baumeister, JCB, 2013 Applications: dynamic in vitro systems Architecture of polyribosomes SNARE mediated membrane fusion Tomography allows us to get 3D snapshots of samples that are not easily accessible by single particle analysis. Brandt et al, Cell, 2009 Applications: Asymmetric viruses Ebola virus HIV-1 Herpesvirus Briggs et al, PNAS 2009 Grunewald et al, Science 2003 Applications: Cells and cellular