沸石固定化细胞降解氰化物的试验研究-高校化学工程学报.PDF

第19 卷第4 期 高 校 化 学 工 程 学 报 No.4 Vol.19 2005 年 8 月 Journal of Chemical Engineering of Chinese Universities Aug. 2005 文章编号：1003-9015(2005)04-0532-04 沸石固定化细胞降解氰化物的实验研究 刘幽燕, 何玉财, 李青云, 童张法 （广西大学化学化工学院, 广西 南宁 530004 ） 摘 要：利用本实验室保藏的一株产碱杆菌DN25作为降氰菌株，以沸石为载体进行固定化，研究了固定化细胞的 降解特性。实验表明采用吸附生长法能有效实现菌株DN25在沸石上固定化，生物固定量可达到0.228g干细胞·(g zeolite)− 1 。固定化细胞的最适降解温度为30 ℃，最适pH 为8.0 ，与游离细胞基本一致。将固定化细胞分别用于50 − − 1 − − 1 mgCN ·L 和500 mgCN ·L 的含氰液的降解，发现对于低浓度含氰废水固定化细胞的初始降解率仅为游离细胞的一 半，而对于高浓度氰废水，固定化细胞的降解速率与游离细胞基本相同。固定化细胞可重复使用10天，降解率仍 能达到90% 。 关键词：氰化物; 废水; 生物降解; 沸石; 固定化 中图分类号：X702.1 ；Q814.2 ；Q93 文献标识码：A Biodegradation of Cyanide by Immobilized Cells on the Zeolite LIU You-yan, HE Yu-cai, LI Qing-yun TONG Zhang-fa (School of Chemistry and Chemical Engineering, Guangxi University, Nanning 530004, China) Abstract: The cyanide-biodegradation characteristics of the immobilized Alcaligenes sp DN25, isolated from contaminated soil and immobilized on the zeolite as carrier was investigated. The results show that the immobilization could be realized through the growth of the cell on the zeolite during the culture process and the biomass immobilized can reach 0.228 g dry weight·(g zeolite)− 1. For the immobilized cell, the optimum biodegradation temperature and pH are 30 ℃ and 8.0 respectively, which are almost similar to that of the free cell. The biodegradation curves of the simulated wastewater containing 50 mg CN— − 1 — − 1 ·L and 500 mg CN ·L respectively were measured and it is found that the initial degradati