核糖体印记技术简介.pdf

Investigate Translational Control via Ribosome Profiling Gene CompanyGene Company 范丹青 Translation? Remember the Central Dogma of Molecular Biology: amino acid -polypeptide-protein Protein levels are a function of: •• RNA stabilityRNA stability • Protein stability hnRN -mRN • Regulation of translation Regulation ? Epigenetics Molecular chaperones RBP Silencing Translation? simple rules about the nature of translation •Ribosome combine with 5’cap •ORFs have a minimum length •Start at the first AUG •Biased codon usage Exceptions •IRES; TEE •initiation at non-AUG codons •leaky scanning •translational reinitiation •translational frameshifts What is Ribosome profiling? Protein synthesis Ribosome translating mRNRibosome translating mRN mRN Ribosome profiling: isolate and sequence only the mRNA protected/actively translated by the ribosome Why is Ribosome Profiling Important? How can you tell how many flowers would be there via the number of buds? No, you can not. Why is Ribosome Profiling Important? High translation Poor tr