中药粉气悬浮臭氧逆流接触灭菌方法研究040511(定稿投中国药学杂志).doc

中药粉灭菌用于中药粉的灭菌采用灭菌选择山茱萸六味药材的原生药粉流通蒸汽灭菌干热灭菌60Co辐射灭菌和臭氧灭菌样品分别灭菌前后X射线衍射（XRD）和红外光谱（IR）进行进行聚类分析化学成分，样品标成分丹皮酚的含量损失率臭氧灭菌法对样品的灭菌率平均为95.24%，比干热灭菌法高6.81%，稍低于60Co辐照灭菌法和灭菌法对化学成分的影响较小，其优越性于灭菌法干热灭菌法。臭氧灭菌效果稳定成分较小中药大生产中对入药生药原粉在线灭菌。 Study on Application of the Air-floating Countercurrent-contacted Sterilization of TCM Powder by Ozone Technology Song Li-li1, Fan Bing-yi1, Gu Chuan-dong2, Lu Peng-wei1, He Ying1, Zhang Qi-ming1, Zhang Yu1, Yang Ke1, Zhu Xin-Ke1 (1 Henan University, Henan Kaifeng,475001,china;2 Zhongren Industrial and Commerical Company,Henan Zhengzhou,450047,china) Abstract Objective: To apply ozone technology in the sterilization of the TCM (Traditional Chinese Medicine) powder. Methods: By means of air-floating ozone countercurrent-contacted sterilization, the powders of crude Sharen and Doukou etc. and the mixture powder of Liuweidihuang pills and so on were sterilized by ozone，the sterilization effects of which were observed, and the processing conditions of which was selected. Six raw powders in Liuweidihuang pills were sterilized respectively by dry-heating, circulating steam, 60Co and ozone. The chemical components were analyzed before and after sterilization by means of XRD and IR, and the results were clustered as well, to probe into their variations of the chemical components. The decreasing rate of Paeonol and Ursolic acid were compared. At the same time, the sterilization efficiency was conducted statistics and comparison. Results: The average sterilization rate of the samples by ozone sterilization method was 95.24%, 6.81% higher than that sterilized by dry-heating，and slightly lower than that sterilized by 60Co radioaction and circulating steam. Ozone sterilization method had no obvious influence on the chemical components of raw powders. Ozone had some advantages over dry-heating and circulating steam method in the sterilization efficiency. Conclusions: Ozone is characterized with a stable sterilization effect and no obvious destoryage. At the same tim