黑葡萄穗霉高环境适应性-应用与环境生物学报.PDF

2017-02-25 DOI: 10.3724/SP.J.1145.2016.01032 / Chin J Appl Environ Biol 2017 23 ( 1 ) : 0117-0122 应用与环境生物学报 ， 黑葡萄穗霉高环境适应性* 张梦雪 杜雨珊 徐圣阳 张小梅** 青岛农业大学资源与环境学院 , 青岛市农村环境工程研究中心 青岛 266109 摘 要 为探究纤维素降解菌黑葡萄穗霉的环境适应机制，分别将该属菌株S05培养于不同pH、不同碳源培养基中，检 测其生长及胞外糖苷水解酶表达情况，并利用糖苷水解酶活性电泳展示各主要酶组分的多样性及其催化性质. 结果 显示，黑葡萄穗霉 S05生长pH范围较宽，在pH 4.5-9.5范围内均能生长，且更适于中性偏碱性环境；胞外主要糖苷水解 pH pH 6.5-8.5 pH 酶组分最适 多为 ， 稳定性较强；该菌株具有精细的表达调控体系，能及时根据环境条件的变化调整酶 组分的组成. 综上表明黑葡萄穗霉 S05的高环境适应性与其胞外糖苷水解酶组分的性质密切相关，对其酶组分的深 入研究可为寻找工业所需中性及偏碱性纤维素酶的来源提供可能. （图4 表 1 参32） 关键词 黑葡萄穗霉；糖苷水解酶；活性电泳；中性纤维素酶 CLC Q939.9 Study on the strong environment adaptiveness of Stachybotrys chartarum* ** ZHANG Mengxue, DU Yushan, XU Shengyang ZHANG Xiaomei Qingdao Engineering Research Center for Rural Environment, College of Resource and Environment, Qingdao Agricultural University, Qingdao 266109, China Abstract This work aimed to find out the reasons for the wide distribution and strong environment adaptiveness of the cellulose-degrading strains of Stachybotrys chartarum. We analyzed the growth state and expression of glycoside hydrolases by cultivating strain S05 of S. chartarum in cellulose media with different pH values or supplemented with different substrates as the sole carbon source. The diversity and the intrinsic properties of glycoside hydrolases were demonstrated through Native- PAGE. The S. chartarum strain S05 exhibited adaptiveness in a wide pH range of pH 4.5-9.5, more adaptive to neutral or alkali environments. It secreted a wide range of glycoside hydrolases, in which the main c