β-淀粉样前体蛋白转基因小鼠发育过程中海马 CAI区神经细胞凋亡变化.pdfVIP

CHINESEJOURNALOFANAT()MYVoL31No．62008 p一淀粉样前体蛋白转基因小鼠发育过程中 海马CAl区神经细胞凋亡变化。 牛艳丽 张维娟 吴 萍于东明 邓锦波△ (河南大学神经生物学研究所，开封475004) 摘要 目的：探讨瑞典家系p～淀粉样前体蛋白(APPSWE)转基因小鼠发育过程中海马CAl区神经细胞凋亡规律。方法： 体细胞形态，免疫组织化学方法观察海马细胞内Caspase-3表达变化，RT-PCR检测Caspase-3mRNA表达变化。结果：随 的发生、发展具有联系。 关键词阿尔茨海默病；海马；p一淀粉样前体蛋白；转基因小鼠；凋亡 of nlolEe’ olangeof删todsin、developinghippocan平us prtxawsorprotein价阴曜|eIlic Ii-amyloid Niu Yanli，ZhangWeijuan，WuPing，Yu Jinbo△ Dongming，Deng (Institute 475004，China) ofNeurobiology，HenanUniversity，Kaifeng Abstract observetheruleof in of mice withAlzheimer Objective：To neuroapoptosisdevelopinghippocampustransgenic disease(APPSWE)．Methods：WildandAPPSWE micefrom 1 tO 180werein- type transgenic postnatalday postnatalday eluded．Nissl andRT—PCRwereusedfor den- immunohistochemistry the P14，the staining，Caspase-3 study．Results：From AD cellsinCAlareaof modelwas withthatof results sityof。positive obviouslyhighercompared age-matchedcontrols；the of werecoincidentwiththedatafrom Caspase-3irnmunohistoehemistry RT-PCR．Conclusion：Theand productiondevelop— mentof diseasebe the of AlzheimermayrelevanttO neuroapoptosisdevelopinghippocampus． wordsAlzheimer Key disease；hippoeampus；辟-amyloidprecurso