胚胎干细胞潜能性的转录调控网络.pdf

Resource An Extended Transcriptional Network for Pluripotency of Embryonic Stem Cells Jonghwan Kim,1,3,4 Jianlin Chu,1,3 Xiaohua Shen,1,3 Jianlong Wang,1,3 and Stuart H. Orkin1,2,3,4,* 1Department of Pediatric Oncology 2Children’s Hospital and Dana Farber Cancer Institute, Harvard Stem Cell Institute 3Harvard Medical School 4Howard Hughes Medical Institute Boston, MA 02115, USA *Correspondence: stuart_orkin@ DOI 10.1016/j.cell.2008.02.039 SUMMARY to a pluripotent state (Park et al., 2007; Takahashi et al., 2007; Yu et al., 2007). How pluripotency is established and maintained in Much attention has focused on a small set of tran- ES cells is of great interest, as an improved understanding of the scription factors that maintain human or mouse transcription factors and epigenetic modifications operating in embryonic stem (ES) cells in a pluripotent state. To a regulatory network will facilitate both directed programming gain a more complete understanding of the regula- of ES cells to specific lineages and the reprogramming of tory network that maintains this state, we identified somatic cells to an ES-like state. target promoters of nine transcription factors, includ- Until recently, attention has focused almost exclusively on a small set of transcription factors, Oct4, Sox2, and Nanog as ing somatic cell reprogramming factors (Oct4, Sox2, ‘‘core’’ pluripotency factors for human or mouse ES cells (Orkin, Klf4, and c-Myc) and others (Nanog, Dax1, Rex1, 2005