磁分离仪分离磁性细菌的新方法.PDF

生 物 工 程 学 报 Chin J Biotech 2010, February 25; 26(2): 270–275 Chinese Journal of Biotechnology ISSN 1000-3061 cjb@ ©2010 CJB, All rights reserved. 生物技术与方法 磁分离仪分离磁性细菌的新方法 刘新星，郭宁，杨英杰，梁万洁，张剑 中南大学资源加工与生物工程学院，长沙 410083 摘 要: 磁性细菌胞内可以产生磁性颗粒，因此具有趋磁性，基于这种特性，利用磁分离的原理，本研究开发了一种 磁性细菌分离仪，提供了一种分离磁性细菌的新方法。以氧化亚铁硫杆菌为例，使用磁性细菌分离仪进行分离，可以 得到强磁菌和弱磁菌。利用透射电镜观察，强磁菌胞内磁性颗粒明显多于弱磁菌；半固体平板磁泳实验也表明强磁菌 趋磁性明显强于弱磁菌。各项实验结果表明磁性细菌分离仪可以有效地分离磁性细菌，这是一种分离磁性细菌的新方 法，将促进磁性细菌分离培养的研究。 关键词: 磁性细菌，细菌磁分离，磁性细菌分离仪 Separation of magnetic bacteria by using a magnetic separator Xinxing Liu, Ning Guo, Yingjie Yang, Wanjie Liang, and Jian Zhang School of Minerals Processing and Bioengineering, Central South University, Changsha 410083, China Abstract: A magnetic separator was used to separate magnetic bacteria based on their magnetotactic characteristics. Acidithiobacillus ferrooxidans , a bacterium that could synthesize intra-cellular nanometer magnetic particles, was investigated as an example. Strong magnetic and weak magnetic cells were separated and collected. On average, the number of the magnetic particles present in the strong magnetic cells is more than that of the weak magnetic cells. Moreover, semisolid-plate magnetophoresis showed that the magnetotaxis of strong magnetic cells was stronger than the weak magnetic cells. These results suggest that the magnetic separator can be used to isolate the magnetic bacteria, which will facilitate the research of magnetic bacteria. Keywords: magnetic bacteria, magnetic separation of bacteria, magnetic separator 氧化亚铁硫杆菌是生物冶金[1] 中的重要细菌之 磁性颗粒。经反复研究发现，氧化亚铁硫杆菌体内 一，能以亚铁、硫元素以及低价硫化物作为能源， 的磁性颗粒数量不多，且没有成链排布，因此其趋 为好氧菌，培养条件简单。本课题组在研究磁黄铁 磁性比较弱。本研究把所有胞内含有天然生成的磁 矿选育浸矿细菌的过程中发现氧化亚铁硫杆菌胞内