激光扫描共聚焦显微镜观察水稻双受精过程.PDF

OI牶牨牥牣牨牭牴牫牫牤j牣cnki牣牨牥牥牬牠牫牪牰牳牣牪牥牥牱牣牥牰牣牥牥牱 河南农业科学 代西梅,黄群策,李国平, 胡秀明,秦广雍 ( , 450052) :利用激光扫描共聚焦显微镜对水稻双受精过程进行了观察。结果表明:开花 0. 5h 可见花 粉粒在柱头上萌发, 形成 一定长度的花粉管,但仍未进入胚囊;开花 1 ～2h 花粉管进入胚囊,但 尚未释放精细胞,此时,在卵器内有两条肌动蛋白冠的形成, 肌动蛋白冠起始于助细胞的基部, 1条 终止于卵细胞核的位置, 另一条终止于极核附近;开花 2～3h 花粉管释放精细胞, 精细胞在肌动 蛋白冠的作用下分别进入极核及卵细胞;开花 3 ～4h, 精核的染色质向卵核扩散,极核内的雄性 核仁逐渐变大, 最 增大至与极核的核仁差不多大小;开花 4 ～5h, 精核与极核完成受精作用,形 成初生胚乳核, 而此时在卵核内出现1 个雄性核仁;开花 6h 左右, 卵核核仁仍未完成与精核核 仁的融合, 此时初生胚乳核已分裂1～2 次;开花 12h, 精核核仁与卵核核仁融合形成1 个较大的 核仁,至此合子形成;开花 24h,合子有丝分裂 1次, 形成2 个细胞的原胚,此时初生胚乳核已分 裂形成8～16 个游离核, 绕胚囊周缘分布。水稻双受精作用属于有丝分裂前配子融合类型。 :水稻;胚囊;双受精;激光扫描共聚焦显微镜 :S511　　:A 　　:1004-3268(2007)06 -0025-06 Observation on the Process of ouble Fertilization in Rice Using Laser Scanning Confocal Microscopy AI X-i mei, HUANG Qun-ce, LI Guo-ping, HU Xiu-ming, QIN Guang-yong (Henan Provincial Key Laboratory of Ion Beam Bi-o engineering, Zhengzhou University,Zhengzhou 450052, China) Absrtact:The processes of double fertilization in rice were observed using laser scanning confocal microscopy.T he results showed that, in 0. 5 hour after flowering, pollen with two sperm cells germinated in the stamen;in 1- 2 hours,pollen tube reached to the embryo sac of ovule. Mean- while, two actin coronas were formed in the ovule. Coronas started from the base of the syner- - gid, one of which ended near the egg nucleus and the other near the p olar nuclei. After 2 3 hours of flowering, the sperm cells were released at the site of the degenerated synergid. When sperm cells were deposited near the base of the corona, sperm cells in the synergid subsequently - migrated toward the point of fusion. In 3 4 ho