动员大鼠自体骨髓干细胞修复损伤心肌-中国医科大学学报.pdfVIP

中国医科大学学报 第43卷 第7期 2014年7月 JournalofChinaMedicalUniversity Vol.43 No.7 Jul.2014 ·621 · ·论著 · 动员大鼠自体骨髓干细胞修复损伤心肌 1 2 1 1 王勃 ，佟文姝 ，李雯娜 ，郭潇繁 （中国医科大学附属第一医院1. 心血管内科；2. 心血管检查科，沈阳 110001） 摘要 目的 探讨应用rhG⁃CSF 促进骨髓干细胞原位移植对损伤心肌的修复作用。方法 采用异丙基肾上腺素腹腔注射建立 大鼠急性心肌损伤模型；通过外源性细胞因子rhG⁃CSF 动员骨髓干细胞增殖、释放、迁移归巢至损伤心肌局部；应用HE 染色结 合图像分析系统，对两组心肌进行对比性分析；应用八导记录仪检测心脏功能。结果 原位移植组的心肌损伤面积小于对照 组；原位移植组心脏收缩功能较对照组明显改善。结论 腹腔注射异丙基肾上腺素可复制急性心肌损伤模型；应用骨髓干细 胞动员剂rhG⁃CSF ，可促进坏死心肌修复，提高心脏收缩功能。 关键词 骨髓干细胞；急性心肌损伤；rhG⁃CSF 中图分类号 R541 文献标志码 A 文章编号 0258-4646 （2014 ）07-0621-04 网络出版地址 MobilizationofAutologousBoneMarrowStemCellstoRepairMyocardialInjuryinRat 1 2 1 1 WANG Bo ，TONG Wen⁃shu ，LI Wen⁃na ，GUO Xiao⁃fan （1. Department of Cardiology，The First Hospital ，China Medical University ，Shenyang 110001，China；2. Department of Cardiovascular Ultrasound，The First Hospital ， China Medical University ，Shenyang 110001，China） Abstract Objective To investigate the repair effect for in⁃situ transplantation of bone marrow stem cells for acute myocardial injury in rats. Meth⁃ ods Acute myocardial injury model was established by intraperitoneal injection of isoproterenol 10 mg/kg in rat. Autologous bone marrow stem cells were mobilized by cytokine ，released and homed to local injured myocardium. A comparative analysis on pathological section between two groups was performed by HE staining and image analysis system. Heart functions were determined by eight lead physiological polygraph system. Results The myocardial necrosis area in in ⁃situ transplantation group was less than that of the control group. The cardiac systolic function was significantly im⁃ proved in in⁃situ transplantation group compared to the control group. Conclusion Acute myocardial injury model could be established by