L-赖氨酸的故事：从代谢途径到工业生产-2017年11月.docVIP

1 L-赖氨酸的故事：从代谢途径到工业生产 1 Introducti导言 2Lysine Biosynthetic P赖氨酸合成途径 33作为生产Corynebacterium glutamicum as a Production Organ谷氨酸棒杆菌 3.1 3.1 赖氨酸合成 Lysine Biosynthes 33.2中碳代谢 Central Carbon Metaboli 3.3 3.3最大赖氨酸生产能力 Maximal Lysine Production Capacity44工程 Strain Engineering 4.1 4.1经典工程 Classical Engineering 4.2 4.2赖氨酸合成的代谢工程 Metabolic Engineering of Lysine Biosynthesis 4.3 4.3 NADPH的代谢工程 Metabolic Engineering of NADPH Metabolism 4.4 4.4代谢工程 Metabolic Engineering of Precursor Supply 4.5 4.5通过系统方法Global Strain Engineering through Systems Biotechnology Approaches55工业生产过程 Industrial Production Processes 5.1 5.1大规模Large-Scale Manufacturing 5.2 5.2工艺优化 Process OptimizationAbstract l-lysine is an essential amino acid required for nutrition of animals and hu-摘要L-赖氨酸是一种动物和所需maIt has to be present in food and feed, which, in many cases, is reali它在食品和饲料，在很多情况下，The high importance of lysine in nu-在营养需求上，赖氨酸的重要性As an important milestone, the glutamate producing soil bacterium Corynebacterium glu-并很快生产another amino acid stemming from the TCA cycle: lysinTCA循环产生的另一种氨基酸：赖氨酸第一次lysine producing strains were obtained.获得赖氨酸生产菌株。 The past 50 years following the discovery of 在50年工业生产菌株开发和优化。This has resulted in effective biotechnological processes currently used for producing目前生物技术生产过程中使用 about 750000 tons of lysine per year.750000吨。 Today, systems-oriented approaches aiming at in- 今天，面向系统的法旨在vestigating the link between the different components of cellular physiology such as细胞不同组成部如transcriptome, fluxome and metabolome, provide a novel powerful platform that w转录，和代谢组分的联系，提供了一个新的强大的平台，将surely drive future research towards holistic understanding of lysine over-producing mi-推动全面的理解赖氨酸优越的生产菌株。 11导言 Introduction The essential amino acid l-lysine is one of the most important amino acids作为动物饲料添加剂L-赖氨酸是最重要的必需氨基酸之一applied as supplement in animal feed.。 Animal feed, which is typically base动物饲料，通常是基于on corn, wheat or barley, is poor in lysin玉米，小麦或大麦，赖氨酸。丰富的赖氨酸The supplementation of such feed到饲料在此之外直