环介导等温扩增技术检测转基因组分研究进展.pdf

5 3 Vol. 5 No. 3 2014 3 Journal of Food Safety and Quality Mar. , 2014 邢宇俊, 吴季荣, 徐剑宏, 史建荣* (, — , , 210014) 摘 要: , , , , , (LAMP), , LAMP , 关键词: ; ; Research progress of LAMP technology in detection of genetically modified component * XING Yu-Jun, WU Ji-Rong, XU Jian-Hong, SHI Jian-Rong (Institute of Food Quality Safety and Detection , Jiangsu Academy of Agricultural Sciences/Key Lab of Food Quality and Safety of Jiangsu Province-State Key Laboratory Breeding Base/Key Laboratory of Control Technology and Standard for Agro-product Safety and Quality , Ministry of Agriculture , Jiangsu Center for GMO Evaluation and Detection , Nanjing 210014, China) ABSTRACT: As more and more genetically modified (GM) crops are approved for commercialization and planting, transgenic product security issues have been to the objection, therefore, regardless of the consumer or government regulators, the development of rapid detection method for genetically modified components is re- quired. Detection of genetically modified component are usually based on nucleic acid detection, and loop-mediated isothermal amplification (LAMP) is one isothermal nucleic acids amplification technique. It has the characteristics of high specificity, sensitivity, operator simple, low cost, visualization results and does